| Stability and analysis of eicosanoids and docosanoids in tissue culture media. | |
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MedLine Citation:
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PMID: 21236355 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Bioactive lipids derived from polyunsaturated fatty acids constitute an important set of lipid mediators with diverse homeostatic and pathophysiological activities. The role of bioactive lipids in inflammation, active resolution of inflammation, reproduction, cardiovascular biology, chronic diseases such as cancer and metabolic disorders is an active area of research. Other than the few clinically used eicosanoids such as prostaglandin F(2)α, prostacyclin, and prostaglandin E(2), little is known about the stability of a majority of the bioactive lipids in aqueous solutions despite their extensive use under in vitro experimental conditions. The present study evaluated the stability of eicosanoids and docosanoids as well as their parent polyunsaturated fatty acids in tissue culture media with respect to incubation time, temperature, and composition of the media using multiple reaction monitoring (MRM) LC-MS. The results provide a comprehensive and valuable data set on the stability of these bioactive lipid mediators to guide in vitro experimentation. |
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Authors:
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Krishna Rao Maddipati; Sen-Lin Zhou |
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Publication Detail:
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Type: Journal Article Date: 2011-01-12 |
Journal Detail:
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Title: Prostaglandins & other lipid mediators Volume: 94 ISSN: 1098-8823 ISO Abbreviation: Prostaglandins Other Lipid Mediat. Publication Date: 2011 Feb |
Date Detail:
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Created Date: 2011-02-02 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9808648 Medline TA: Prostaglandins Other Lipid Mediat Country: United States |
Other Details:
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Languages: eng Pagination: 59-72 Citation Subset: IM |
Copyright Information:
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Copyright © 2011 Elsevier Inc. All rights reserved. |
Affiliation:
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Department of Pathology, Wayne State University School of Medicine, Detroit, MI, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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