Document Detail

Stability after thawing of RBCs frozen with the high- and low-glycerol method.
MedLine Citation:
PMID:  12559010     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: RBCs can be frozen with either the high-glycerol method (HGM) or the low-glycerol method (LGM). To date, the use of frozen RBCs is hampered by a 24-hour outdating period after thawing. A closed washing system (ACP 215) may solve this problem. STUDY DESIGN AND METHODS: We compared the effects of high- (40%) and low-glycerol (19%) concentration, with and without freezing (at -80 degrees C for HGM, -196 degrees C for LGM) on the in vitro quality of RBCs after deglycerolization with the closed washing system and during storage at 4 degrees C in SAGM after thawing. RESULTS: Glycerol treatment by itself induced hemolysis during processing, which was more pronounced in HGM cells. The freeze-thaw-wash process decreased the stability of RBCs, particularly in LGM cells during storage after thawing. In contrast to LGM cells, in HGM cells no additional effect of freeze or thaw on stability of washed cells was seen during the first week of storage after thawing. Changes in osmotic resistance and cellular metabolism could not explain the observed differences in RBC stability. CONCLUSION: The closed washing system is able to process both high- and low-glycerol-treated RBCs. Stability after washing during cold storage in SAGM, as measured by hemolysis, is better for HGM cells as compared to LGM cells.
Charles C M Lelkens; Femke Noorman; Jack G Koning; Rosa Truijens-de Lange; Perry S Stekkinger; Joa C Bakker; Johan W M Lagerberg; Anneke Brand; Arthur J Verhoeven
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Transfusion     Volume:  43     ISSN:  0041-1132     ISO Abbreviation:  Transfusion     Publication Date:  2003 Feb 
Date Detail:
Created Date:  2003-01-31     Completed Date:  2003-04-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0417360     Medline TA:  Transfusion     Country:  United States    
Other Details:
Languages:  eng     Pagination:  157-64     Citation Subset:  IM    
Military Blood Bank, Leiden, The Netherlands.
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MeSH Terms
Blood Preservation / methods*,  standards
Cryopreservation / methods*,  standards
Cryoprotective Agents / pharmacology*
Dose-Response Relationship, Drug
Erythrocyte Transfusion / standards
Erythrocytes / cytology*
Glycerol / pharmacology*
Hemolysis / drug effects
Reg. No./Substance:
0/Cryoprotective Agents; 56-81-5/Glycerol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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