Document Detail

Stability of Steviol Glycosides in Several Food Matrices.
MedLine Citation:
PMID:  23020306     Owner:  NLM     Status:  Publisher    
As steviol glycosides are now allowed as a food additive in the European market, it is important to assess the stability of these steviol glycosides after they have been added to different food matrices. We analyzed and tested the stability of steviol glycosides in semi-skimmed milk, soy drink, fermented milk drink, ice cream, full-fat and skimmed set yoghurt, dry bis-cuits and jam. The fat was removed by centrifugation from the dairy and soy drink samples. Proteins were precipitated by addition of acetonitrile and also removed by centrifugation. Samples of jam were extracted with water. Dry biscuits were extracted with ethanol. The resulting samples were concentrated with solid phase extraction and analysed by HPLC on a C18 stationary phase and a gradient of acetonitrile / aqueous 25 mM phosphoric acid. The accuracy was checked using a standard addition on a number of samples from each matrix. For assessing the stability of the steviol glycosides, samples were stored in conditions relevant to each food matrix and analyzed periodically. The results indicate that steviol glycosides can be analyzed with good precision and accuracy in these food categories. The recovery was between 96 and 103 %. The method was also validated by standard addition, which showed excellent agreement with the external calibration curve. No sign of decomposition of steviol glycosides was found in any of the samples.
Etienne Jooken; Ruis Amery; Tom Struyf; Barbara Duquenne; Jan Mc Geuns; Boudewijn Meesschaert
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-9-29
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  -     ISSN:  1520-5118     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-10-1     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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