| SrfJ, a Salmonella type III secretion system effector regulated by PhoP, RcsB, and IolR. | |
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MedLine Citation:
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PMID: 22661691 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Virulence-related type III secretion systems are present in many Gram-negative bacterial pathogens. These complex devices translocate proteins, called effectors, from the bacterium into the eukaryotic host cell. Here, we identify the product of srfJ, a Salmonella enterica serovar Typhimurium gene regulated by SsrB, as a new substrate of the type III secretion system encoded by Salmonella pathogenicity island 2. The N-terminal 20-amino-acid segment of SrfJ was recognized as a functional secretion and translocation signal specific for this system. Transcription of srfJ was positively regulated by the PhoP/PhoQ system in an SsrB-dependent manner and was negatively regulated by the Rcs system in an SsrB-independent manner. A screen for regulators of an srfJ-lacZ transcriptional fusion using the T-POP transposon identified IolR, the regulator of genes involved in myo-inositol utilization, as an srfJ repressor. Our results suggest that SrfJ is synthesized both inside the host, in response to intracellular conditions, and outside the host, in myo-inositol-rich environments. |
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Authors:
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Mar Cordero-Alba; Joaquín Bernal-Bayard; Francisco Ramos-Morales |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2012-06-01 |
Journal Detail:
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Title: Journal of bacteriology Volume: 194 ISSN: 1098-5530 ISO Abbreviation: J. Bacteriol. Publication Date: 2012 Aug |
Date Detail:
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Created Date: 2012-07-30 Completed Date: 2012-10-16 Revised Date: 2013-04-08 |
Medline Journal Info:
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Nlm Unique ID: 2985120R Medline TA: J Bacteriol Country: United States |
Other Details:
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Languages: eng Pagination: 4226-36 Citation Subset: IM |
Affiliation:
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Departamento de Genética, Facultad de Biología, Universidad de Sevilla, Seville, Spain. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Artificial Gene Fusion Bacterial Proteins / genetics, metabolism* Bacterial Secretion Systems / genetics* Gene Expression Profiling Gene Expression Regulation, Bacterial* Genes, Reporter Membrane Proteins / genetics, metabolism Microfilament Proteins / metabolism* Salmonella typhimurium / genetics, metabolism* Transcription Factors / metabolism Virulence Factors / genetics, metabolism* beta-Galactosidase / genetics, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/Membrane Proteins; 0/Microfilament Proteins; 0/SPI-2 protein, Salmonella; 0/Transcription Factors; 0/Virulence Factors; 125360-99-8/PhoP protein, Bacteria; 127737-30-8/RcsB protein, Bacteria; EC 3.2.1.23/beta-Galactosidase |
| Comments/Corrections | |
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