Document Detail

Src interacts with dynamin and synapsin in neuronal cells.
MedLine Citation:
PMID:  9539797     Owner:  NLM     Status:  MEDLINE    
The nonreceptor tyrosine kinase Src is expressed at a high level in cells that are specialized for regulated secretion, such as the neuron, and is concentrated on secretory vesicles or at the site of exocytosis. To investigate the possibility that Src may play a role in regulating membrane traffic, we searched for neuronal proteins that will interact with Src. The SH3 domain of Src, but not that of the splice variant N-Src, bound to three proteins from mouse synaptosomes or PC12 cells: dynamin, synapsin Ia, and synapsin Ib. Dynamin and the synapsins coprecipitated with Src from PC12 cell extracts, and they colocalized with a subset of Src in the PC12 cell by immunofluorescence. Neither dynamin nor the synapsins were phosphorylated by Src, suggesting that the interaction of these proteins serves to direct the kinase activity of Src toward other proteins in the vesicle population. In immunoprecipitates containing Src and dynamin, the clathrin adaptor protein alpha-adaptin was also found. The association of Src and synapsin suggests a role for Src in the life cycle of the synaptic vesicle. The identification of a complex containing Src, dynamin, and alpha-adaptin indicates that Src may play a more general role in membrane traffic as well.
A Foster-Barber; J M Bishop
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  95     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1998 Apr 
Date Detail:
Created Date:  1998-05-13     Completed Date:  1998-05-13     Revised Date:  2013-04-17    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  4673-7     Citation Subset:  IM    
G. W. Hooper Foundation, University of California, San Francisco, CA 94143-0552, USA.
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MeSH Terms
3T3 Cells
Binding Sites
Cell Differentiation
Cell Membrane / metabolism
Cells, Cultured
GTP Phosphohydrolases / isolation & purification,  metabolism*
Mice, Knockout
Neurons / cytology,  metabolism*
PC12 Cells
Synapsins / isolation & purification,  metabolism*
src Homology Domains
src-Family Kinases / isolation & purification,  metabolism*
Grant Support
Reg. No./Substance:
0/Synapsins; EC Kinases; EC 3.6.1.-/GTP Phosphohydrolases; EC

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