Document Detail


Squeezing and detachment of living cells.
MedLine Citation:
PMID:  21112279     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The interaction of living cells with their environment is linked to their adhesive and elastic properties. Even if the mechanics of simple lipid membranes is fairly well understood, the analysis of single cell experiments remains challenging in part because of the mechanosensory response of cells to their environment. To study the mechanical properties of living cells we have developed a tool that borrows from micropipette aspiration techniques, atomic force microscopy, and the classical Johnson-Kendall-Roberts test. We show results from a study of the adhesion properties of living cells, as well as the elastic response and relaxation. We present models that are applied throughout the different stages of an experiment, which indicate that the contribution of the different components of the cell are active at various stages of compression, retraction, and detachment. Finally, we present a model that attempts to elucidate the surprising logarithmic relaxation observed when the cell is subjected to a given deformation.
Authors:
Marie-Josée Colbert; Françoise Brochard-Wyart; Cécile Fradin; Kari Dalnoki-Veress
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biophysical journal     Volume:  99     ISSN:  1542-0086     ISO Abbreviation:  Biophys. J.     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-11-29     Completed Date:  2011-03-07     Revised Date:  2013-07-03    
Medline Journal Info:
Nlm Unique ID:  0370626     Medline TA:  Biophys J     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3555-62     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Biophysical Society. Published by Elsevier Inc. All rights reserved.
Affiliation:
Department of Physics & Astronomy and the Brockhouse Institute for Materials Research, McMaster University, Hamilton, Ontario, Canada.
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MeSH Terms
Descriptor/Qualifier:
Biomechanics
Cell Adhesion
Cells / cytology*
Elasticity*
HeLa Cells
Humans
Stress, Mechanical
Time Factors
Comments/Corrections

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