Document Detail

Squamous differentiation downregulates Muc1 mucin in hamster tracheal surface epithelial cell.
MedLine Citation:
PMID:  10814515     Owner:  NLM     Status:  MEDLINE    
To investigate whether the squamous differentiation of primary hamster tracheal epithelial cell, which is induced by retinoic acid deficiency or chronic PMA treatment, regulates Muc1 expression, we first produced and characterized a monoclonal antibody against hamster tracheal Muc1 mucin using pGEX-Muc1 fusion protein as an antigen and the changes of Muc1 mucin expression was determined by Western blot. Squamous differentiation downregulated the expression of Muc1 mucin from HTSE cells. The decrease in the immunoreactivity of Muc1 mucin was parallel to the decrease in the immunoreactivity of high molecular weight mucin, which is secreted from HTSE cells. The data from the present study implicate a possible role of Muc1 mucin in squamous differentiation of HTSE cells.
C Y Shin; K H Park; B K Ryu; E Y Choi; K C Kim; K H Ko
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  271     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2000 May 
Date Detail:
Created Date:  2000-06-20     Completed Date:  2000-06-20     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  641-6     Citation Subset:  IM    
Copyright Information:
Copyright 2000 Academic Press.
Department of Pharmacology, Seoul National University, Seoul, 151-742, Korea.
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MeSH Terms
Antibodies, Monoclonal / pharmacology
Blotting, Western
Cell Differentiation
Cells, Cultured
Enzyme-Linked Immunosorbent Assay
Gene Expression Regulation / genetics
Mucin-1 / genetics,  immunology,  metabolism*
Recombinant Fusion Proteins / metabolism
Trachea / metabolism*
Tretinoin / pharmacology
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Mucin-1; 0/Recombinant Fusion Proteins; 302-79-4/Tretinoin

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