Document Detail

Spreading dynamics and wetting transition of cellular aggregates.
MedLine Citation:
PMID:  21504944     Owner:  NLM     Status:  MEDLINE    
We study the spreading of spheroidal aggregates of cells, expressing a tunable level of E-cadherin molecules, on glass substrates decorated with mixed fibronectin and polyethylene glycol. We observe the contact area by optical interferometry and the profile by side-view microscopy. We find a universal law of aggregate spreading at short times, which we interpret through an analogy with the spreading of viscoelastic droplets. At long times, we observe either partial wetting or complete wetting, with a precursor film of cells spreading around the aggregate with two possible states. In strongly cohesive aggregates this film is a cellular monolayer in the liquid state, whereas in weakly cohesive aggregates, cells escape from the aggregate, forming a 2D gas. The escape of isolated cells is a physical mechanism that appears also to be present in the progression of a noninvasive tumor into a metastatic malignant carcinoma, known as the epithelial-mesenchymal transition.
Stéphane Douezan; Karine Guevorkian; Randa Naouar; Sylvie Dufour; Damien Cuvelier; Françoise Brochard-Wyart
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Publication Detail:
Type:  Journal Article     Date:  2011-04-19
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  108     ISSN:  1091-6490     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  2011 May 
Date Detail:
Created Date:  2011-05-04     Completed Date:  2011-07-15     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  7315-20     Citation Subset:  IM    
Unité Mixte de Recherche 168, and Unité Mixte de Recherche 144, Institut Curie, Centre National de la Recherche Scientifique, Université Pierre et Marie Curie, 26 rue d'Ulm, 75248 Paris cedex 05, France.
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MeSH Terms
Cadherins / metabolism*
Cell Adhesion / physiology*
Cell Movement / physiology*
Cells, Cultured
Interferometry / methods
Polyethylene Glycols
Reg. No./Substance:
0/Cadherins; 0/Fibronectins; 0/Polyethylene Glycols

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