Document Detail


Spread of a single multiresistant methicillin-resistant Staphylococcus aureus clone carrying a variant of staphylococcal cassette chromosome mec type III isolated in a university hospital.
MedLine Citation:
PMID:  17180608     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The purpose of the study was the molecular characterization of methicillin-resistant Staphylococcus aureus (MRSA) isolates cultured from patients treated in seven wards of a university hospital in Lublin, Poland, over a 14-month period. Eleven nosocomial MRSA isolates were analyzed. Phenotypic identification of the isolates as MRSA was confirmed by the detection of the nuc and mecA genes using a multiplex PCR assay. The MRSA isolates were further characterized by pulsed-field gel electrophoresis, 16S-23S rRNA spacer length polymorphism analysis, and the simplex and multiplex SCCmec PCR assays. The MRSA isolates were found to be multiresistant: in addition to resistance to beta-lactam agents, they demonstrated resistance to ciprofloxacin, tetracycline, erythromycin, and gentamicin. The MRSA isolates were genetically identical and shared common pulsed-field gel electrophoresis profiles and 16S-23S rRNA spacer length polymorphism profiles. The PCR-based method revealed that the profile of the Lublin clone was identical to that of the Brazilian pandemic MRSA isolates. By SCCmec typing, all MRSA isolates harbored the C variant of the SCCmec type III that differed from the typical SCCmec type III pattern by the lack of locus F (414 bp). The results of this study indicate the spread of a single, multiresistant, MRSA clone in various wards of a university hospital over a 14-month period. The SCCmec structure harbored by the Lublin clone has previously been identified among Polish MRSA isolates representing the HoMRSA-Pol1 clone. The data from this study indicate that the Lublin MRSA clone is most probably genetically related to the HoMRSA-Pol1 clone. Moreover, this latter clone belongs to ST239, the same sequence type as the Hungarian and Brazilian pandemic MRSA isolates.
Authors:
A Szczepanik; M Kozioł-Montewka; Z Al-Doori; D Morrison; D Kaczor
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology     Volume:  26     ISSN:  0934-9723     ISO Abbreviation:  Eur. J. Clin. Microbiol. Infect. Dis.     Publication Date:  2007 Jan 
Date Detail:
Created Date:  2007-01-11     Completed Date:  2007-03-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8804297     Medline TA:  Eur J Clin Microbiol Infect Dis     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  29-35     Citation Subset:  IM    
Affiliation:
Department of Clinical Microbiology, Medical University of Lublin, Lublin, Poland. agola7@wp.pl
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MeSH Terms
Descriptor/Qualifier:
Bacterial Proteins / genetics*
Bacterial Typing Techniques
Cross Infection / epidemiology,  microbiology*
Disease Outbreaks*
Drug Resistance, Multiple / genetics
Drug Resistance, Multiple, Bacterial
Electrophoresis, Gel, Pulsed-Field
Endonucleases / genetics*
Hospitals, University
Humans
Methicillin Resistance / drug effects,  genetics
Micrococcal Nuclease / genetics*
Poland / epidemiology
RNA, Ribosomal, 16S
Staphylococcal Infections / genetics*
Staphylococcus aureus / drug effects*,  genetics*,  pathogenicity
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/RNA, Ribosomal, 16S; 0/mecA protein, Staphylococcus aureus; 0/nuc protein, staphylococcus; EC 3.1.-/Endonucleases; EC 3.1.31.1/Micrococcal Nuclease

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