Document Detail


Spontaneous release of histamine from basophils and histamine-releasing factor in patients with atopic dermatitis and food hypersensitivity.
MedLine Citation:
PMID:  2473400     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Patients with hypersensitivity to food documented by a double-blind, placebo-controlled oral food challenge have been reported to have a high rate of release of histamine from basophils in vitro. To determine whether patients with atopic dermatitis and food hypersensitivity had similar high rates of spontaneous histamine release in vitro, whether dietary elimination of relevant food antigens affected this release, and whether a cytokine, histamine-releasing factor, could account for it, we evaluated 63 patients with atopic dermatitis and food hypersensitivity (38 of whom had eliminated the offending foods from their diets), 20 patients with atopic dermatitis without food hypersensitivity, and 18 normal volunteers. Patients with atopic dermatitis and food hypersensitivity were found to have higher rates of spontaneous release of histamine from basophils than controls (mean +/- SE, 35.1 +/- 3.9 percent vs. 2.3 +/- 0.2 percent; P less than 0.001). Those who had eliminated the offending food allergen from the diet for an extended period had a significantly lower rate of histamine release (3.7 +/- 0.5 percent; P less than 0.001). In patients with atopic dermatitis without food hypersensitivity, the rate (1.8 +/- 0.2 percent) did not differ from that in normal controls. Mononuclear cells from persons with food allergies spontaneously produced a histamine-releasing factor in vitro that provoked the release of histamine from the basophils of other food-sensitive persons, but not from those of normal controls. Patients who adhered to a restricted diet had a decline in the rate of spontaneous generation of the factor by their mononuclear cells. The histamine-releasing factor was found to activate basophils through surface-bound IgE. We conclude that in patients with food hypersensitivity, exposure to the relevant antigens produces a cytokine (histamine-releasing factor) that interacts with IgE bound to the surface of basophils, causing them to release histamine.
Authors:
H A Sampson; K R Broadbent; J Bernhisel-Broadbent
Publication Detail:
Type:  Clinical Trial; Controlled Clinical Trial; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The New England journal of medicine     Volume:  321     ISSN:  0028-4793     ISO Abbreviation:  N. Engl. J. Med.     Publication Date:  1989 Jul 
Date Detail:
Created Date:  1989-08-16     Completed Date:  1989-08-16     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0255562     Medline TA:  N Engl J Med     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  228-32     Citation Subset:  AIM; IM    
Affiliation:
Department of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, MD 21205.
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Basophils / immunology*
Child
Child, Preschool
Dermatitis, Atopic / immunology*
Double-Blind Method
Food Hypersensitivity / immunology*
Histamine Release*
Humans
Leukocytes, Mononuclear / immunology
Lymphokines / physiology*
Tumor Markers, Biological*
Grant Support
ID/Acronym/Agency:
AI 24439/AI/NIAID NIH HHS; AI00830/AI/NIAID NIH HHS; RR-00052/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Lymphokines; 0/Tumor Markers, Biological; 0/translationally controlled tumor protein, p23
Comments/Corrections
Comment In:
N Engl J Med. 1989 Jul 27;321(4):255-7   [PMID:  2473401 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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