Document Detail

Spontaneous osteoclast formation from peripheral blood mononuclear cells in postmenopausal osteoporosis.
MedLine Citation:
PMID:  15611151     Owner:  NLM     Status:  MEDLINE    
Osteoclasts are cells involved in bone reabsorbing and hence in postmenopausal bone loss. There is no evidence of increased in vitro spontaneous osteoclast formation in postmenopausal osteoporosis. The aim of our study was to evaluate spontaneous osteoclastogenesis in osteoporosis. Bone mineral density, markers of bone turnover, and cultures of peripheral blood mononuclear cells (PBMC) on dentine slices with or without the addition of 1,25-OH vitamin D3 ([10(-8) M]) were obtained from 18 osteoporotic women and 15 controls. To verify cytokine production by PBMC cultures, supernatants were collected on days 3 and 6 and tested for TNF-alpha and RANKL. The data obtained were compared between patients and controls by one-way ANOVA and correlated by Pearson's coefficient. We found a significant increase in osteoclast formation and bone reabsorbing activity in patients with respect to controls; in addition, the production of TNF-alpha and RANKL is significantly higher in patients. Furthermore, osteoclast number is inversely correlated with bone mineral density and directly with RANKL in culture supernatants. Our data demonstrated an increased spontaneous osteoclastogenesis in women affected by postmenopausal osteoporosis: this increase may be explained by the higher production of TNF-alpha and RANKL by PBMC cultures of osteoporotic patients.
Patrizia D'Amelio; Anastasia Grimaldi; Gian Piero Pescarmona; Cristina Tamone; Ilaria Roato; Giancarlo Isaia
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2004-12-20
Journal Detail:
Title:  FASEB journal : official publication of the Federation of American Societies for Experimental Biology     Volume:  19     ISSN:  1530-6860     ISO Abbreviation:  FASEB J.     Publication Date:  2005 Mar 
Date Detail:
Created Date:  2005-03-04     Completed Date:  2005-10-25     Revised Date:  2012-02-15    
Medline Journal Info:
Nlm Unique ID:  8804484     Medline TA:  FASEB J     Country:  United States    
Other Details:
Languages:  eng     Pagination:  410-2     Citation Subset:  IM    
Department of Internal Medicine, Center on Experimental Medicine (CeRMS), University of Torino, Turin, Italy.
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MeSH Terms
Bone Density
Bone Resorption
Calcitriol / pharmacology
Carrier Proteins / biosynthesis,  pharmacology
Cell Count
Cell Differentiation
Cells, Cultured
Leukocytes, Mononuclear / cytology*
Linear Models
Macrophage Colony-Stimulating Factor / pharmacology
Membrane Glycoproteins / biosynthesis,  pharmacology
Osteoclasts / cytology*,  drug effects,  physiology
Osteoporosis, Postmenopausal / blood*
RANK Ligand
Receptor Activator of Nuclear Factor-kappa B
Tumor Necrosis Factor-alpha / biosynthesis
Reg. No./Substance:
0/Carrier Proteins; 0/Membrane Glycoproteins; 0/RANK Ligand; 0/Receptor Activator of Nuclear Factor-kappa B; 0/TNFRSF11A protein, human; 0/TNFSF11 protein, human; 0/Tumor Necrosis Factor-alpha; 32222-06-3/Calcitriol; 81627-83-0/Macrophage Colony-Stimulating Factor

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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