Document Detail


Spontaneous and neurally activated depolarizations in smooth muscle cells of the guinea-pig urethra.
MedLine Citation:
PMID:  9852327     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
1. Membrane potential recordings were made from longitudinal smooth muscle cells of the guinea-pig urethra using conventional microelectrode techniques. 2. Smooth muscle cells of the urethra developed spontaneous transient depolarizations (STDs) and slow waves. Single unit STDs had amplitudes of approximately 5 mV and slow waves seemed to occur as amplitude multiples of single unit STDs. 3. STDs and slow waves were abolished by niflumic acid or low chloride solution and also by cyclopiazonic acid (CPA), BAPTA or high concentrations of caffeine. Lower concentrations of caffeine abolished slow waves but not STDs. Nifedipine inhibited slow waves but not STDs. 4. When stochastic properties of STDs were examined, it was found that the intervals between occurrences were not well modelled by Poisson statistics, instead the STDs appeared to be clustered. 5. Transmural stimulation evoked excitatory junctional potentials (EJPs) and triggered slow waves which were abolished by either alpha,beta-methylene-ATP or tetrodotoxin. Evoked slow waves were also abolished by caffeine, co-application of caffeine and ryanodine or by CPA which left EJPs unaffected. 6. In conclusion, smooth muscle cells of urethra exhibit STDs which are clustered rather than random events, and are the result of spontaneous Ca2+ release from intracellular stores and subsequent activation of Ca2+-activated chloride channels. STDs sum to activate L-type Ca2+ channels which contribute to the sustained phase of slow waves. Stimulation of purinoceptors by neurally released ATP initiates EJPs and also causes the release of Ca2+ from intracellular stores to evoke slow waves.
Authors:
H Hashitani; F R Edwards
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of physiology     Volume:  514 ( Pt 2)     ISSN:  0022-3751     ISO Abbreviation:  J. Physiol. (Lond.)     Publication Date:  1999 Jan 
Date Detail:
Created Date:  1999-03-22     Completed Date:  1999-03-22     Revised Date:  2013-06-11    
Medline Journal Info:
Nlm Unique ID:  0266262     Medline TA:  J Physiol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  459-70     Citation Subset:  IM    
Affiliation:
Department of Zoology, The University of Melbourne, Parkville, Victoria 3052, Australia. hikaru@clyde.its.unimelb.edu.au
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MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphate / analogs & derivatives,  pharmacology
Animals
Caffeine / pharmacology
Egtazic Acid / analogs & derivatives,  pharmacology
Electric Stimulation
Evoked Potentials / drug effects,  physiology*
Female
Guanethidine / pharmacology
Guinea Pigs
Indoles / pharmacology
Membrane Potentials / drug effects,  physiology*
Microelectrodes
Muscle, Smooth / drug effects,  innervation,  physiology*
Neuromuscular Junction / drug effects,  physiology*
Nifedipine / pharmacology
Niflumic Acid / pharmacology
Phenylephrine / pharmacology
Stochastic Processes
Synaptic Transmission / drug effects,  physiology
Tetrodotoxin / pharmacology
Urethra / drug effects,  innervation,  physiology*
Chemical
Reg. No./Substance:
0/Indoles; 21829-25-4/Nifedipine; 4368-28-9/Tetrodotoxin; 4394-00-7/Niflumic Acid; 55-65-2/Guanethidine; 56-65-5/Adenosine Triphosphate; 58-08-2/Caffeine; 59-42-7/Phenylephrine; 67-42-5/Egtazic Acid; 7292-42-4/alpha,beta-methyleneadenosine 5'-triphosphate; K22DDW77C0/1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid; X9TLY4580Z/cyclopiazonic acid
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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