Document Detail


Spontaneous lymphatic vessel formation and regression in the murine cornea.
MedLine Citation:
PMID:  20739466     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: Lymphatic dysfunctions are associated with many diseases, ranging from cancer metastasis to transplant rejection, for which there is little effective treatment. To date, there is no natural model with which to study lymphatic regression. This study was conducted to investigate whether murine cornea, an extensively exploited tissue for vascular studies, derives its lymphatic-free status from a natural regression mechanism. The differential behaviors between the lymphatic and blood vessels under normal development and inflammation conditions are also compared.
METHODS: Normal mouse eyeballs or whole-mount corneas encompassing the entire course of corneal development and maturation and adult inflamed corneas were used for immunofluorescent microscopic studies.
RESULTS: The data demonstrated, for the first time, that mouse cornea was endowed with a significant number of lymphatic vessels that underwent spontaneous formation and regression during a critical period after birth, which was not observed for blood vessels. Because lymphatic growth can be reactivated in the adult cornea after inflammatory stimulation, the cornea thereby becomes the first tissue ever identified to have a full range of lymphatic plasticity.
CONCLUSIONS: These novel findings not only provide a new concept in defining the cornea and its related diseases, they also reveal a completely natural model with which to study both lymphatic regression and formation. It is hoped that further studies will divulge novel and potent pro- or anti-lymphatic factors to treat lymphatic disorders inside and outside the eye.
Authors:
Hui Zhang; Xuemei Hu; Julie Tse; Firehiwott Tilahun; Mengsheng Qiu; Lu Chen
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2011-01-21
Journal Detail:
Title:  Investigative ophthalmology & visual science     Volume:  52     ISSN:  1552-5783     ISO Abbreviation:  Invest. Ophthalmol. Vis. Sci.     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2011-01-24     Completed Date:  2011-02-25     Revised Date:  2011-08-01    
Medline Journal Info:
Nlm Unique ID:  7703701     Medline TA:  Invest Ophthalmol Vis Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  334-8     Citation Subset:  IM    
Affiliation:
Center for Eye Disease and Development, Program in Vision Science and School of Optometry, University of California, Berkeley, California 94720, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Newborn
Antigens, CD31 / metabolism
Antigens, Differentiation / metabolism
Blood Vessels / embryology*,  metabolism
Cornea / embryology*,  growth & development,  metabolism
Endothelium, Lymphatic / embryology,  metabolism
Female
Fluorescent Antibody Technique, Indirect
Glycoproteins / metabolism
Homeodomain Proteins / metabolism
Keratitis / metabolism
Lymphangiogenesis / physiology*
Lymphatic Vessels / embryology*,  metabolism
Male
Mice
Mice, Inbred C57BL
Microscopy, Fluorescence
Neovascularization, Physiologic / physiology*
Pregnancy
Tumor Suppressor Proteins / metabolism
Chemical
Reg. No./Substance:
0/Antigens, CD31; 0/Antigens, Differentiation; 0/Glycoproteins; 0/Homeodomain Proteins; 0/Tumor Suppressor Proteins; 0/Xlkd1 protein, mouse; 0/monocyte-macrophage differentiation antigen; 0/prospero-related homeobox 1 protein
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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