Document Detail

Spontaneous induction of endogenous murine leukemia virus-related antigen expression during short-term in vitro incubation of mouse lymphocytes.
MedLine Citation:
PMID:  4365582     Owner:  NLM     Status:  MEDLINE    
Short-term lymphocyte cultures from mouse thymus, spleen, or lymph nodes were studied for the presence of murine leukemia virus group-specific antigens with an immunofluorescence test using rat immune sera against syngeneic cells infected with the radiation leukemia virus or its pseudotype of murine sarcoma virus and goat and rabbit antisera against purified murine leukemia virus group-specific antigen. Antigens reacting with these sera appeared in the cultured lymphocytes within 24 hr, and the proportion of immunofluorescent-positive cells increased to 25-80% by the second or third day of cultivation, concomitantly with a decrease in cell viability. The appearance of these antigens can be suppressed by inhibitors of DNA (mitomycin-C), RNA (actinomycin-D, cordycepin, and polyadenylic acid), and protein (cycloheximide) synthesis. No infectious virus could be detected by the immunofluorescence and XC-cell tests. The observed phenomenon appears to represent the spontaneous partial derepression of endogenous murine leukemia virus replication in lymphocytes during short-term in vitro cultivation.
P Lonai; A Declève; H S Kaplan
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  71     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1974 May 
Date Detail:
Created Date:  1974-08-23     Completed Date:  1974-08-23     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2008-12     Citation Subset:  IM    
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MeSH Terms
Antigens, Viral / analysis*
Cells, Cultured
Cycloheximide / pharmacology
Cytarabine / pharmacology
Dactinomycin / pharmacology
Fluorescent Antibody Technique
Hybridization, Genetic
Leukemia Virus, Murine / immunology*
Lymph Nodes / immunology*
Lymphocytes / immunology*
Mice, Inbred Strains
Mitomycins / pharmacology
Oligonucleotides / pharmacology
Spleen / immunology*
Thymus Gland / immunology*
Time Factors
Reg. No./Substance:
0/Antigens, Viral; 0/Mitomycins; 0/Oligonucleotides; 147-94-4/Cytarabine; 50-76-0/Dactinomycin; 66-81-9/Cycloheximide

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