Document Detail


Spontaneous immortalisation of ensheathing cells from adult rat olfactory nerve.
MedLine Citation:
PMID:  8833195     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this report, we describe the isolation of a cell line, Rolf B1.T, from cultures of adult rat olfactory nerve cells. Rolf B1.T cells have an antigenic phenotype which closely resembles that of olfactory ensheathing cells. In routine culture conditions, Rolf B1.T cells constitutively express glial fibrillary acidic protein, S1OO, the low-affinity neurotrophin receptor p75 NGF, laminin, tenascin, and the neural cell adhesion molecule (N-CAM); a variable proportion of the cells also express cadherin, which is regulated by local culture conditions and is associated positively with cell proliferation status. We provide evidence that the association may be indirect and linked to a related parameter such as local cell density. Rolf B1.T cells arose from a population of less well-differentiated cells after a spontaneous immortalisation event. The cells retain many characteristics of normal cells, are dependent on serum growth factors for their proliferation, and fail to grow in semi-solid agar. Rolf B1.T cells support the regrowth of neurites from adult retinal ganglion cells in vitro in a heterologous co-culture system and will have potential value in investigations into the mechanisms of glial support for axonal regeneration from adult mammalian central neurons.
Authors:
R J Sonigra; S S Kandiah; C B Wigley
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Glia     Volume:  16     ISSN:  0894-1491     ISO Abbreviation:  Glia     Publication Date:  1996 Mar 
Date Detail:
Created Date:  1997-05-15     Completed Date:  1997-05-15     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8806785     Medline TA:  Glia     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  247-56     Citation Subset:  IM    
Affiliation:
Division of Anatomy and Cell Biology, United Medical and Dental Schools of Guys and St. Thomas's, London, United Kingdom.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigens / analysis
Biological Markers
Cell Division
Cell Line, Transformed
Coculture Techniques
Female
Male
Nerve Regeneration
Neurites / physiology
Olfactory Nerve / cytology*,  immunology,  physiology
Phenotype
Rats
Rats, Wistar
Retinal Ganglion Cells / cytology,  physiology
Chemical
Reg. No./Substance:
0/Antigens; 0/Biological Markers

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