Document Detail

Spontaneous and gamma-ray-induced sister chromatid exchanges in patients with carcinoma of cervix uteri.
MedLine Citation:
PMID:  16019927     Owner:  NLM     Status:  MEDLINE    
The aim was to investigate whether there are differences in the spontaneous and gamma-ray-induced genomic instability in peripheral blood lymphocytes between untreated cervical cancer patients and healthy women using the sister chromatid exchange (SCE) assay as an indicator of chromosomal instability. Lymphocyte cultures from whole venous blood of 10 patients with cervical neoplasia and 10 healthy female volunteers were cultivated in vitro and irradiated using a 60Co-gamma source. Slides were prepared using the standard air-drying procedure and stained by the fluorescence-plus Giemsa (FPG) technique. The number of SCE and the number of chromosomes were assessed in second-division metaphases. A radiation dose-dependent increase of SCE/cell and SCE/chromosome values were found in healthy women as well as in patients, while statistical analysis has shown significantly higher SCE frequencies in healthy women as compared with patients. Cellular kinetics expressed as replication indices (RI) calculated from the frequency of cells in first cell division (M1), second cell division (M2) and third cell division (M3) were also significantly different, while observed RI were higher for patients than for control individuals. The results suggest that patients with carcinoma of the cervix uteri have chromosomal stability changes reflected in statistically different levels of spontaneous and induced SCE in comparison with healthy individuals. Despite the unknown mechanisms of SCE formation, it is felt that the changed SCE frequency, especially after mutagen treatment, may be used as a marker of increased cancer risk.
E Bozsakyová; L Wsólová; I Chalupa
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Publication Detail:
Type:  Clinical Trial; Controlled Clinical Trial; Journal Article    
Journal Detail:
Title:  International journal of radiation biology     Volume:  81     ISSN:  0955-3002     ISO Abbreviation:  Int. J. Radiat. Biol.     Publication Date:  2005 Feb 
Date Detail:
Created Date:  2005-07-19     Completed Date:  2005-08-11     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8809243     Medline TA:  Int J Radiat Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  177-85     Citation Subset:  IM; S    
Cancer Research Institute of Slovak Academy of Sciences, Bratislava, Slovak Republic.
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MeSH Terms
Background Radiation
Case-Control Studies
Cells, Cultured
Dose-Response Relationship, Radiation
Gamma Rays / adverse effects*
Genomic Instability / radiation effects*
Lymphocytes / radiation effects*
Middle Aged
Radiation Dosage
Sister Chromatid Exchange / genetics*,  radiation effects*
Uterine Neoplasms / blood*,  genetics*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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