Document Detail

Spontaneous cell shedding by tumor cells in monolayer culture.
MedLine Citation:
PMID:  3782006     Owner:  NLM     Status:  MEDLINE    
Sarcoma 180 monolayers spontaneously shed single cells and small multicellular aggregates into the surrounding medium to produce a dual population of floating and substratum-attached cells. Shedding was a motility-associated event that occurred when cells attempted to migrate over one another. It resulted from a combination of cell shape change and active motility, which increased sensitivity to fluid shear dislodgement by reducing a cell's surface area of adhesive contact and increasing strain tension at its adhesive contact points. Shedding occurred at all phases of the cell cycle. Extracellular matrix but not conditioned medium enhanced the floating subpopulation by slowing the kinetics of reattachment to plastic and cellular substrate. Although sarcoma 180 cells are anchorage independent in the sense that they grow readily in single cell suspension, they nevertheless exhibited anchorage modulation of their cell cycle. Short periods in suspension produced a mild G1 accumulation, whereas longer periods of anchorage deprivation led to a mild G2 accumulation which appeared to result from an interference with cytokinesis.
P Skehan; J E Thomas; S J Friedman
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  In vitro cellular & developmental biology : journal of the Tissue Culture Association     Volume:  22     ISSN:  0883-8364     ISO Abbreviation:  In Vitro Cell. Dev. Biol.     Publication Date:  1986 Nov 
Date Detail:
Created Date:  1986-12-23     Completed Date:  1986-12-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8506951     Medline TA:  In Vitro Cell Dev Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  632-6     Citation Subset:  IM    
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MeSH Terms
Cell Adhesion
Cell Aggregation
Cell Cycle
Cell Movement
Cells, Cultured
Flow Cytometry
Sarcoma 180 / pathology*,  ultrastructure

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