| Spontaneous and bolus-induced motility in the chronically obstructed guinea-pig small intestine in vitro. | |
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MedLine Citation:
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PMID: 17562174 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Partial obstruction of the small intestine results in dysmotility and morphometric changes proximal to the site of obstruction. However, our understanding of the relation between the morphometric remodeling and change in the motility pattern during chronic obstruction is sparse. The aim of this study was to investigate the effect of partial chronic intestinal obstruction on motility, morphology, and collagen content proximal and distal to the site of obstruction. Twenty guinea-pigs with partial intestinal obstruction and eight sham-operated controls lived for four weeks. Spontaneous and bolus-induced motility was recorded in isolated intestinal segments proximal and distal to the site of obstruction using a perfused low-compliance pressure-measuring system in vitro. After the motility experiments, the specimens were fixed at 2 kPa luminal distension pressure and sampled for histomorphometric determination of luminal radius, layer thickness, and wall thickness. Total wall collagen was also determined. The area under the curve (AUC) of spontaneous contractions and the amplitude, frequency, and AUC for the bolus-induced motility were higher in the proximal segments of the banded animals compared to distal segments and to the intestinal segments in the control animals (P < 0.05). The radius-to-thickness ratio was lowest in the proximal segments of the obstructed animals (P < 0.01). The collagen content was three times higher proximal to the site of obstruction when compared to distal locations and to the controls (P < 0.01). The AUC at 2 ml bolus injections plotted against the radius-to-thickness ratio showed a strong association (r = 0.97 for control, and r = 0.99 for obstruction, P < 0.01). No correlation was found between the collagen content and AUC. In conclusion, partial intestinal obstruction in guinea pigs caused pronounced changes in morphology and motility. An association was found between the radius-to-thickness ratio and bolus-induced motility. |
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Authors:
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Jan Henrik Storkholm; Jingbo Zhao; Gerda E Villadsen; Hans Gregersen |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, N.I.H., Extramural Date: 2007-06-12 |
Journal Detail:
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Title: Digestive diseases and sciences Volume: 53 ISSN: 0163-2116 ISO Abbreviation: Dig. Dis. Sci. Publication Date: 2008 Feb |
Date Detail:
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Created Date: 2008-01-24 Completed Date: 2008-03-27 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7902782 Medline TA: Dig Dis Sci Country: United States |
Other Details:
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Languages: eng Pagination: 413-20 Citation Subset: AIM; IM |
Affiliation:
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Center of Excellence in Visceral Biomechanics and Pain, Aalborg Hospital, DK-9100 Aalborg, Denmark. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Area Under Curve Chronic Disease Collagen / analysis Disease Models, Animal* Gastrointestinal Motility* Guinea Pigs Intestinal Obstruction / pathology, physiopathology* Jejunum / pathology, physiopathology* |
| Grant Support | |
ID/Acronym/Agency:
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R01 DK072616/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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9007-34-5/Collagen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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