Document Detail

Spontaneous action potentials of cells in the canine sinus node.
MedLine Citation:
PMID:  1277407     Owner:  NLM     Status:  MEDLINE    
Right atria were excised from the hearts of 20 young dogs (15 +/- 5 weeks old) and maintained in vitro in order to perform microelectrode impalements. The atria contracted spontaneously for at least 6 hours at a stable rate of 127/min when they were perfused with Krebs-Ringer bicarbonate solution through the sinus node artery. Cells within a small area near the sinus node artery and between the superior vena cava and right atrium consistently yielded transmembrane potentials typical of pacemaker cells. This area was verified histologically to be the midportion of the sinus node. Electrical characteristics of these sinus node pacemaker cells were: maximum diastolic potential, -56 +/- 7 mV (mean +/- 1 SD); action potential amplitude, 56 +/- 8 mV; overshoot, 0 +/- 2 mV. Smooth transitions from phase 4 to phase 0 were always present. From these experiments we conclude that the excised perfused canine right atrium provides a useful new experimental cardiac preparation because it maintains stable spontaneous activity for many hours in vitro and the sinus node within it is readily accessible for study by intracellular microelectrodes.
W T Woods; F Urthaler; T N James
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Circulation research     Volume:  39     ISSN:  0009-7330     ISO Abbreviation:  Circ. Res.     Publication Date:  1976 Jul 
Date Detail:
Created Date:  1976-08-23     Completed Date:  1976-08-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0047103     Medline TA:  Circ Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  76-82     Citation Subset:  IM    
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MeSH Terms
Action Potentials*
Electrophysiology / instrumentation
Membrane Potentials
Perfusion / methods
Sinoatrial Node / cytology,  physiology*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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