| Spontaneous [Ca2+]i oscillations in G1/S phase-synchronized cells. | |
| | |
MedLine Citation:
|
PMID: 19460967 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Ca(2+) signaling controls a wide range of cellular functions such as division, fertilization, apoptosis and necrosis. Specifically, calcium signaling is thought to play a crucial role in driving cells through the different stages of the cell-division cycle. In most cells, however, this fact is far from being established. Few studies have examined this question from a different perspective: whether cells exhibit some characteristic cell cycle-dependent intracellular calcium-signaling patterns. This approach is effective in discerning the causal relationship between Ca(2+) signaling and the cell cycle. Through synchronization of the cell cycle, flow cytometry and confocal scanning microscopic intracellular calcium ion concentration ([Ca(2+)](i)) imaging, the present study shows that the G1/S phase transition is uniquely characterized by spontaneous [Ca(2+)](i) oscillations that last for up to 40 min. Most likely, these oscillations emanate from the [Ca(2+)](i) signaling that accompanies DNA replication as the cell prepares for the next division cycle. These temporal signals further affirm the significance of Ca(2+) in the cell cycle. |
| | |
Authors:
|
Afadhali Denis Russa; Chihaya Maesawa; Yoh-ichi Satoh |
Related Documents
:
|
20074037 - The anaphase-promoting complex/cyclosome (apc/c): cell-cycle-dependent and -independent... 11239447 - Ctla-4 regulates induction of anergy in vivo. 14997017 - Beta-amyloid-activated cell cycle in sh-sy5y neuroblastoma cells: correlation with the ... 17208957 - Cold nights impair leaf growth and cell cycle progression in maize through transcriptio... 8472857 - C-myc gene chromatin of estrogen receptor positive and negative breast cancer cells. 6777297 - Secretion of either of a pair of immunoglobulins, igm or igx, in somatic hybrid cells d... |
Publication Detail:
|
Type: Letter; Research Support, Non-U.S. Gov't Date: 2009-05-21 |
Journal Detail:
|
Title: Journal of electron microscopy Volume: 58 ISSN: 1477-9986 ISO Abbreviation: J Electron Microsc (Tokyo) Publication Date: 2009 Oct |
Date Detail:
|
Created Date: 2009-09-18 Completed Date: 2009-12-01 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 7611157 Medline TA: J Electron Microsc (Tokyo) Country: Japan |
Other Details:
|
Languages: eng Pagination: 321-9 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals COS Cells / cytology, metabolism Calcium / metabolism* Calcium Signaling* Cell Cycle / drug effects Cercopithecus aethiops DNA Replication Flow Cytometry G1 Phase / drug effects*, physiology Hela Cells / cytology, metabolism Humans Microscopy, Confocal S Phase / drug effects*, physiology |
| Chemical | |
Reg. No./Substance:
|
7440-70-2/Calcium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Inhibition of Hedgehog signaling enhances delivery of chemotherapy in a mouse model of pancreatic ca...
Next Document: Comment on "Functional traits and niche-based tree community assembly in an Amazonian forest".