Document Detail


Spoligotyping of multidrug-resistant Mycobacterium tuberculosis isolates in Ethiopia.
MedLine Citation:
PMID:  23317962     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
SETTING: St Peter Tuberculosis (TB) Specialized Hospital and the Aklilu Lemma Institute of Pathobiology, Addis Ababa, Ethiopia.
OBJECTIVE: To genotype multidrug-resistant tuberculosis (MDR-TB) isolates and assess the magnitude of their clustering.
DESIGN: A total of 183 consecutive MDR-TB isolates collected between September 2009 and February 2012 were characterised using molecular typing. Prior to the study, the isolates were confirmed as MDR-TB using GenoType® MTBDRplus. Recent transmission index was used to analyse the clusters.
RESULTS: Spoligotyping identified 43 different patterns, of which 17 consisted of at least two isolates forming clusters, while 26 had only a single isolate. The most frequent patterns were spoligo international typing (SIT) number 21 and 149. Twenty-four patterns did not match existing patterns in the SpolDB4 database. The strains belonged to three lineages, the predominant lineages being Euro-American and Indo-Oceanic, each consisting of 65 isolates. High proportions (86%) of patients were infected with clustered strains, suggesting probable recent transmission of MDR-TB in the study area.
CONCLUSION: The observation of cluster formation of the spoligotype patterns of MDR-TB isolates could suggest transmission of MDR-TB strains among the population, thus warranting further attention.
Authors:
B Diriba; T Berkessa; G Mamo; Y Tedla; G Ameni
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease     Volume:  17     ISSN:  1815-7920     ISO Abbreviation:  Int. J. Tuberc. Lung Dis.     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-01-15     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9706389     Medline TA:  Int J Tuberc Lung Dis     Country:  France    
Other Details:
Languages:  eng     Pagination:  246-50     Citation Subset:  IM    
Affiliation:
St Paul's Hospital Millennium Medical College, Federal Ministry of Health, Addis Ababa, Ethiopia; Aklilu Lemma Institute of Pathobiology, Addis Ababa University, Addis Ababa, Ethiopia.
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