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Splitting of porcine embryos at the four-cell or morula stage.
MedLine Citation:
PMID:  16727449     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
Porcine embryos obtained from estrus-induced prepuberal gilts were split at the 4-cell and morula stage. The in vitro development of the demi-embryos was compared with that of intact control embryos. The intact control embryos developed according to expectation. The in vitro survival of the demi-embryos was inferior to that of the respective controls. The splitting of 4-cell embryos was easier to accomplish and more efficient than the splitting of morulae. The in vitro development of the 4-cell embryos was also slightly better, although this difference was not significant. In vitro development of demi-embryos originating from morulae split at room temperature was slightly although nonsignificantly inferior to that of demi-embryos from morulae split on a warming stage at 37 degrees C. Between 14 and 18 demi-embryos were transferred to synchronous recipient gilts after 24 to 48 h of culture. Of 3 gilts receiving split 4-cell embryos, 1 gilt aborted 2 normal fetuses on Day 90 of pregnancy and 1 carried a single piglet to term. Of the 4 gilts receiving split morulae, 1 gilt had 4 normal and 3 degenerated fetuses upon slaughter on Day 35 of pregnancy.
Authors:
J Stein-Stefani; W Holtz
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Theriogenology     Volume:  41     ISSN:  0093-691X     ISO Abbreviation:  Theriogenology     Publication Date:  1994  
Date Detail:
Created Date:  2006-05-26     Completed Date:  2009-12-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0421510     Medline TA:  Theriogenology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  961-8     Citation Subset:  -    
Affiliation:
Institute of Animal Husbandry and Genetics, Göttingen University, Albrecht-Thaer-Weg 3, Göttingen, Germany.
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