Document Detail


Splenorenal reflex modulates renal blood flow in the rat.
MedLine Citation:
PMID:  15090605     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have previously shown that the splenorenal reflex controls renin release through splenic afferent and renal sympathetic nerves. We proposed that this reflex would also affect renal blood flow (RBF). RBF was measured in male Long Evans rats using transit-time flow probes. There were no significant differences between any of the experimental groups with respect to baseline values of RBF (8.9 +/- 0.4 ml min(-1), n= 25) or mean arterial pressure (MAP, 98.7 +/- 2.5 mmHg, n= 25). Splenic venous pressure was selectively raised (from 7.9 +/- 0.6 to 21.6 +/- 0.3 mmHg, n= 25) in anaesthetized rats by partial ligation of the splenic vein. This caused an immediate fall in RBF (-2.1 +/- 0.2 ml min(-1), n= 7) and in MAP (-12.4 +/- 2.8 mmHg, n= 7). The fall in RBF, but not the fall in blood pressure, was attenuated by renal denervation (DeltaRBF: - 0.7 +/- 0.1 ml min(-1), n= 6), splenic denervation (DeltaRBF: -0.8 +/- 0.1 ml min(-1), n= 6) and close renal arterial injection of the alpha1-adrenergic blocker phenoxybenzamine (12.5 microg; DeltaRBF: -0.8 +/- 0.1 ml min(-1), n= 6). Renal conductance fell only in the intact control group, i.e. the residual fall in RBF in the denervated and phenoxybenzamine-treated animals could be attributed to the fall in MAP. We also showed that splenic vein occlusion increased both splenic afferent (from 3.0 +/- 0.3 to 6.6 +/- 0.6 spikes s(-1), n= 5) and renal efferent (from 24.8 +/- 2.0 to 50.2 +/- 4.9 spikes s(-1), n= 9) nerve activity. We conclude that obstruction to splenic venous outflow, such as would occur in portal hypertension, initiates increased splenic afferent nerve activity and renal vasoconstriction through the splenorenal reflex, as well as a fall in blood pressure. We propose that this contributes to the renal and cardiovascular dysfunction observed in portal hypertension.
Authors:
Shereen M Hamza; Susan Kaufman
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2004-04-16
Journal Detail:
Title:  The Journal of physiology     Volume:  558     ISSN:  0022-3751     ISO Abbreviation:  J. Physiol. (Lond.)     Publication Date:  2004 Jul 
Date Detail:
Created Date:  2004-06-30     Completed Date:  2005-01-19     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0266262     Medline TA:  J Physiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  277-82     Citation Subset:  IM    
Affiliation:
Department of Physiology, University of Alberta, Edmonton, Alberta, Canada T6G 2S2.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blood Pressure / physiology
Denervation
Male
Neurons, Afferent / physiology
Neurons, Efferent / physiology
Rats
Rats, Long-Evans
Receptors, Adrenergic, alpha-1 / physiology
Reflex / physiology*
Renal Circulation / physiology*
Renin / blood
Spleen / blood supply,  innervation*,  physiology
Splenic Vein / physiology
Venous Pressure / physiology
Chemical
Reg. No./Substance:
0/Receptors, Adrenergic, alpha-1; EC 3.4.23.15/Renin
Comments/Corrections

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