Document Detail

Splenic hemodynamics and decreased endothelial nitric oxide synthase in the spleen of rats with liver cirrhosis.
MedLine Citation:
PMID:  17481668     Owner:  NLM     Status:  MEDLINE    
The enlarged spleen in liver cirrhosis is considered to play a role in the pathogenesis of portal hypertension, but the splenic hemodynamics and molecular mechanisms behind the phenomenon have not been elucidated. The present study aimed to examine the splenic hemodynamics associated with splenic microcirculation and congestion, and to determine the status of the endothelial nitric oxide synthase (eNOS) signaling pathway in the spleen of rats with liver cirrhosis. Liver cirrhosis was induced by bile duct ligation. In rats with bile duct ligation (BDL rats) and control rats, splenic blood flow was measured using a laser Doppler flowmeter, and splenic blood volume was measured using a near-infrared spectrophotometer. The expressions of eNOS and its upstream effectors, Akt, TNF-alpha and VEGF, in the spleen were also determined. Specific splenic blood flow was significantly decreased in BDL rats compared with control rats. Specific splenic blood volume was also decreased in BDL rats, while their total splenic blood volume, especially the deoxygenated volume, was significantly increased. The expressions of phosphorylated and total eNOS, and the eNOS phosphorylation ratio, were all significantly decreased in the spleen of BDL rats. The Akt phosphorylation ratio and TNF-alpha concentration were also decreased in the spleen of BDL rats although the expression of VEGF was increased. These findings suggest that the eNOS signaling pathway is suppressed in the spleen of cirrhotic rats, and may contribute to the measured decreases in specific blood flow and volume in the spleen of liver cirrhosis. Determination of the factors influencing the suppression of eNOS in the spleen may shed light on how liver cirrhosis results in hypodynamic intrasplenic circulation.
Shohei Yamaguchi; Hirofumi Kawanaka; Daisuke Yoshida; Yoshihiko Maehara; Makoto Hashizume
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-03-23
Journal Detail:
Title:  Life sciences     Volume:  80     ISSN:  0024-3205     ISO Abbreviation:  Life Sci.     Publication Date:  2007 May 
Date Detail:
Created Date:  2007-05-08     Completed Date:  2007-06-20     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0375521     Medline TA:  Life Sci     Country:  England    
Other Details:
Languages:  eng     Pagination:  2036-44     Citation Subset:  IM    
Department of Disaster and Emergency Medicine, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
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MeSH Terms
Blood Flow Velocity
Hypertension, Portal / etiology
Laser-Doppler Flowmetry
Liver Cirrhosis, Experimental / complications,  enzymology*,  physiopathology*
Nitric Oxide Synthase Type III / metabolism*
Proto-Oncogene Proteins c-akt / metabolism
Rats, Sprague-Dawley
Signal Transduction
Spleen / blood supply*
Tumor Necrosis Factor-alpha / metabolism
Vascular Endothelial Growth Factor A / metabolism
Reg. No./Substance:
0/Tumor Necrosis Factor-alpha; 0/Vascular Endothelial Growth Factor A; EC Oxide Synthase Type III; EC Proteins c-akt

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