Document Detail

Spleen neoangiogenesis in patients with myelofibrosis with myeloid metaplasia.
MedLine Citation:
PMID:  14871248     Owner:  NLM     Status:  MEDLINE    
Neoangiogenesis is an integral component of bone marrow myeloproliferation in patients with myelofibrosis with myeloid metaplasia (MMM). As extramedullary haematopoiesis is a constitutive feature of MMM, we studied spleen neoangiogenesis by a computerized image analysis in MMM patients. Compared with five normal subjects, spleen CD34-staining capillary vascular density (CVD) was 2.1-3.03 times higher than the upper range of normal in six of the 15 (40%) MMM patients. CD8-staining sinusoidal vascular density (SVD) was constantly normal or lesser than normal and was inversely correlated with CVD (R = -0.53; P = 0.04). In MMM patients who did not receive cytoreductive or radiation therapy in the month before splenectomy (n = 9), the CVD was a significant determinant of spleen size (R = 0.88; P = 0.04). In MMM patients, the number of spleen CD34+ haematopoietic stem cells was increased from 1.2 to 98 times the upper limit of normal, and predicted the expansion of CVD (R = 0.57; P = 0.03). A population of cells expressing the CD34+/CD133+/VEGFR-2+ angiopoietic phenotype was present in the blood and spleen of five of seven patients. These results document that neoangiogenesis is an integral component of spleen re-localization of haematopoietic stem cells and suggest a cellular mechanism for spleen neoangiogenesis.
Giovanni Barosi; Vittorio Rosti; Margherita Massa; Gian Luca Viarengo; Alessandro Pecci; Vittorio Necchi; Isabella Ramaioli; Rita Campanelli; Monia Marchetti; Mario Bazzan; Umberto Magrini
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  British journal of haematology     Volume:  124     ISSN:  0007-1048     ISO Abbreviation:  Br. J. Haematol.     Publication Date:  2004 Mar 
Date Detail:
Created Date:  2004-02-11     Completed Date:  2004-05-10     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0372544     Medline TA:  Br J Haematol     Country:  England    
Other Details:
Languages:  eng     Pagination:  618-25     Citation Subset:  IM    
Laboratory of Medical Informatics, IRCCS Policlinico S. Matteo, Viale Golgi 19, 27100 Pavia, Italy.
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MeSH Terms
Antigens, CD34 / analysis
Middle Aged
Neovascularization, Pathologic / blood,  complications,  pathology*
Primary Myelofibrosis / blood,  complications,  pathology*
Spleen / blood supply*
Vascular Endothelial Growth Factor A / blood
Reg. No./Substance:
0/Antigens, CD34; 0/Vascular Endothelial Growth Factor A
Erratum In:
Br J Haematol. 2004 Jul;126(2):284-5

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