| Splanchnic endotoxin levels in cirrhotic rats induced by carbon tetrachloride. | |
| | |
MedLine Citation:
|
PMID: 10746415 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
BACKGROUND: Bacterial translocation (passage of intestinal bacteria to mesenteric lymph nodes) observed in cirrhosis may be a source of endotoxin that can stimulate nitric oxide production and participate in the pathogenesis of hyperdynamic circulation. Currently, there are no published data concerning splanchnic endotoxin levels in cirrhotic rats. This study was designed to determine systemic and portal hemodynamics and to detect endotoxins in the portal and systemic circulation. METHODS: Liver cirrhosis was induced by carbon tetrachloride intragastric gavage. Systemic and splanchnic endotoxin levels in control rats and cirrhotic rats with or without ascites were measured using a chromogenic Limulus assay. In addition, systemic and portal hemodynamic data were obtained using a thermodilution technique and catheterization. RESULTS: Cirrhotic rats with ascites had the lowest systemic vascular resistance (2.6 +/- 0.1 mmHg.ml-1.min.100 g body weight, BW) compared with control rats (6.3 +/- 0.3 mmHg.ml-1.min.100 g BW; p < 0.05) and cirrhotic rats without ascites (3.7 +/- 0.3 mmHg.ml-1.min.100 g BW; p < 0.05). Cirrhotic rats with ascites displayed the highest splanchnic levels of endotoxin (10.6 +/- 3.1 pg/ml) compared with cirrhotic rats without ascites (2.0 +/- 0.7 pg/ml; p < 0.05) and control rats (2.0 +/- 0.4 pg/ml; p < 0.05). There was no difference in the splanchnic endotoxin levels between control rats and cirrhotic rats without ascites (p > 0.05). Similar results were observed with systemic endotoxin values (cirrhotic rats with ascites, 10.8 +/- 2.8 pg/ml; cirrhotic rats without ascites, 2.7 +/- 0.6 pg/ml; control rats, 2.5 +/- 0.4 pg/ml; p < 0.05). A significant correlation existed between portal and systemic endotoxin values in cirrhotic rats with or without ascites (r = 0.96, p < 0.001 and r = 0.9, p < 0.05, respectively), whereas this correlation did not exist in control rats (r = 0.5, p > 0.05). CONCLUSIONS: Cirrhotic rats with ascites had the lowest systemic vascular resistance and the highest splanchnic endotoxin levels when compared with cirrhotic rats without ascites and control rats. These results suggest that splanchnic endotoxemia may be involved in the development and/or maintenance of hyperdynamic circulation. |
| | |
Authors:
|
C J Chu; F Y Lee; S S Wang; F Y Chang; H C Lin; R H Lu; C C Chan; S D Lee |
Related Documents
:
|
15739185 - Combined endostatin/sflt-1 antiangiogenic gene therapy is highly effective in a rat mod... 172145 - Size and charge heterogeneity of rat tissue ferritins. 3923485 - Purification, induction, and distribution of placental glutathione transferase: a new m... 2333015 - Phosphatidylcholine synthesis in the rat: the substrate for methylation and regulation ... 21720005 - Correlation between hyper-sensitivity to hydrogen peroxide and low defense against ca(2... 8706205 - Augmented sensitivity to benzodiazepine in septic shock rats. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Zhonghua yi xue za zhi = Chinese medical journal; Free China ed Volume: 63 ISSN: 0578-1337 ISO Abbreviation: Zhonghua Yi Xue Za Zhi (Taipei) Publication Date: 2000 Mar |
Date Detail:
|
Created Date: 2000-04-27 Completed Date: 2000-04-27 Revised Date: 2007-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0005327 Medline TA: Zhonghua Yi Xue Za Zhi (Taipei) Country: CHINA |
Other Details:
|
Languages: eng Pagination: 196-204 Citation Subset: IM |
Affiliation:
|
Department of Medicine, Taipei Veterans General Hospital, Taiwan, ROC. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Ascites / physiopathology Carbon Tetrachloride Endotoxins / blood* Hemodynamics / drug effects Liver Cirrhosis, Experimental / physiopathology* Male Rats Rats, Sprague-Dawley Splanchnic Circulation* Vascular Resistance / drug effects |
| Chemical | |
Reg. No./Substance:
|
0/Endotoxins; 56-23-5/Carbon Tetrachloride |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Sudomotor abnormalities in reflex sympathetic dystrophy.
Next Document: Endovascular embolization of intractable epistaxis.