Document Detail

Splanchnic Th(2) and Th(1) cytokine redistribution in microsurgical cholestatic rats.
MedLine Citation:
PMID:  20031157     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Long-term extrahepatic cholestasis in the rat induces ductular proliferation and fibrosis in the liver, portal hypertension, splenomegaly, portosystemic collateral circulation, and ascites. These splanchnic alterations could have an inflammatory pathophysiology. MATERIAL AND METHODS: We measured serum levels of hepatobiliary injury markers and the acute phase proteins, alpha-1-major acid protein (alpha(1)-MAP) and alpha-1-acid glycoprotein (alpha(1)-GPA) in rats 6 wk after microsurgical extrahepatic cholestasis. We also assayed Th(1) (TNF-alpha and IL-1beta) and Th(2) (IL-4 and IL-10) cytokine levels in the liver, ileum, spleen, and mesenteric lymph complex by enzyme-linked immunosorbent assay (ELISA) techniques. Liver fibrosis was measured by Sirius red stain and by using an image system computer-assisted method and mast cell liver infiltration by Giemsa stain. RESULTS: The cholestatic rats showed an increase (P<0.001) in serum levels of bile acids, total and direct bilirubin, AST, ALT, AST/ALT index, gamma-GT, alkaline phosphatase, alpha(1)- MAP, alpha(1)-GPA, and LDH (P<0.05) in relation to sham-operated rats. TNF-alpha, IL-1beta, IL-4, and IL-10 increased in the ileum (P<0.01) and mesenteric lymph complex (P<0.001), and decreased in the liver (P<0.001). A marked bile proliferation associated with fibrosis (P<0.001) and mast cell infiltration was also shown in the liver of cholestatic rats. CONCLUSION: The splanchnic redistribution of cytokines, with an increase of Th(1) and Th(2) production in the small bowel and in the mesenteric lymph complex, supports the key role of inflammatory mechanisms in rats with secondary biliary fibrosis.
José García-Dominguez; María-Angeles Aller; Cruz García; Felipe de Vicente; Maria-Teresa Corcuera; Fernando Gómez-Aguado; María José Alonso; Elena Vara; Jaime Arias
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-11-11
Journal Detail:
Title:  The Journal of surgical research     Volume:  162     ISSN:  1095-8673     ISO Abbreviation:  J. Surg. Res.     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-07-23     Completed Date:  2010-09-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0376340     Medline TA:  J Surg Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  203-12     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Elsevier Inc. All rights reserved.
Plastic Surgery Unit, Getafe University Hospital, Madrid, Spain.
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MeSH Terms
Blood Pressure
Body Weight
Cholestasis / surgery*
Collateral Circulation / physiology*
Enzyme-Linked Immunosorbent Assay
Ileum / metabolism
Interleukin-10 / metabolism
Interleukin-1beta / metabolism
Interleukin-4 / metabolism
Liver / metabolism
Liver Cirrhosis / pathology
Liver Function Tests
Mesenteric Veins / physiopathology
Microsurgery / methods
Organ Size
Portal Vein / physiopathology
Rats, Wistar
Splanchnic Circulation
Spleen / metabolism
Tumor Necrosis Factor-alpha / metabolism
Reg. No./Substance:
0/Interleukin-1beta; 0/Tumor Necrosis Factor-alpha; 130068-27-8/Interleukin-10; 207137-56-2/Interleukin-4

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