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Spiroplasma diabroticae sp. nov., from the southern corn rootworm beetle, Diabrotica undecimpunctata (Coleoptera:Chrysomelidae).
MedLine Citation:
PMID:  8995806     Owner:  NLM     Status:  MEDLINE    
Spiroplasma strain DU-1T (T = type strain), which was isolated from hemolymph of the corn rootworm Diabrotica undecimpunctata (Coleoptera:Chrysomelidae), was serologically distinct from other spiroplasma species, groups, and subgroups. Cells of strain DU-1T were shown by light microscopy to be helical motile filaments. Electron microscopy revealed cells bounded by a single cytoplasmic membrane, with no evidence of a cell wall. The organism was not sensitive to 500 U of penicillin per ml. Strain DU-1T grew well in SM-1, M1D, and SP-4 liquid media, in broth supplemented with 1% bovine serum fraction or conventional horse serum, and under both aerobic and anaerobic conditions. This organism did not appear to have a sterol requirement for growth, as has been reported for several other Spiroplasma species or strains. Optimal growth occurred at 32 degrees C, with a doubling time of 0.9 h; strain DU-1T multiplied at 10 to 41 degrees C but failed to grow at 5 or 43 degrees C. It produced acid from glucose but hydrolyzed neither arginine nor urea. The results of reciprocal serologic tests in which antigens or antisera to established Spiroplasma species, groups, subgroups, and putative groups were used indicated that strain DU-1T was serologically distinct. This organism has a DNA guanine-plus-cytosine content of 25 +/- 1 mol% and a genome size of 1,350 kbp. Strain DU-1T is a member of a cluster of fast-growing insect-associated spiroplasmas, as determined by sequence analysis of 16S rRNA. On the basis of the results of this study and previously published data, strain DU-1 (= ATCC 43210) is designated the type strain of a new species, Spiroplasma diabroticae.
P Carle; R F Whitcomb; K J Hackett; J G Tully; D L Rose; J M Bove; R B Henegar; M Konai; D L Williamson
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  International journal of systematic bacteriology     Volume:  47     ISSN:  0020-7713     ISO Abbreviation:  Int. J. Syst. Bacteriol.     Publication Date:  1997 Jan 
Date Detail:
Created Date:  1997-02-13     Completed Date:  1997-02-13     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0042143     Medline TA:  Int J Syst Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  78-80     Citation Subset:  IM    
Laboratoire de Biologie Cellulaire et Moléculaire, Institut Nationale de Recherche Agronomique, Villenave d'Ornon, France.
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MeSH Terms
Arginine / metabolism
Bacteriological Techniques
Base Composition
Cross Reactions / immunology
Culture Media / metabolism
DNA, Bacterial / analysis
Glucose / metabolism
Microbial Sensitivity Tests
Microscopy, Electron
Molecular Sequence Data
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Spiroplasma / classification*,  genetics,  immunology,  ultrastructure
Sterols / metabolism
Urea / metabolism
Reg. No./Substance:
0/Culture Media; 0/DNA, Bacterial; 0/RNA, Ribosomal, 16S; 0/Sterols; 50-99-7/Glucose; 57-13-6/Urea; 74-79-3/Arginine

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