Document Detail

Spiral-structured, nanofibrous, 3D scaffolds for bone tissue engineering.
MedLine Citation:
PMID:  19642211     Owner:  NLM     Status:  MEDLINE    
Polymeric nanofiber matrices have already been widely used in tissue engineering. However, the fabrication of nanofibers into complex three-dimensional (3D) structures is restricted due to current manufacturing techniques. To overcome this limitation, we have incorporated nanofibers onto spiral-structured 3D scaffolds made of poly (epsilon-caprolactone) (PCL). The spiral structure with open geometries, large surface areas, and porosity will be helpful for improving nutrient transport and cell penetration into the scaffolds, which are otherwise limited in conventional tissue-engineered scaffolds for large bone defects repair. To investigate the effect of structure and fiber coating on the performance of the scaffolds, three groups of scaffolds including cylindrical PCL scaffolds, spiral PCL scaffolds (without fiber coating), and spiral-structured fibrous PCL scaffolds (with fiber coating) have been prepared. The morphology, porosity, and mechanical properties of the scaffolds have been characterized. Furthermore, human osteoblast cells are seeded on these scaffolds, and the cell attachment, proliferation, differentiation, and mineralized matrix deposition on the scaffolds are evaluated. The results indicated that the spiral scaffolds possess porosities within the range of human trabecular bone and an appropriate pore structure for cell growth, and significantly lower compressive modulus and strength than cylindrical scaffolds. When compared with the cylindrical scaffolds, the spiral-structured scaffolds demonstrated enhanced cell proliferation, differentiation, and mineralization and allowed better cellular growth and penetration. The incorporation of nanofibers onto spiral scaffolds further enhanced cell attachment, proliferation, and differentiation. These studies suggest that spiral-structured nanofibrous scaffolds may serve as promising alternatives for bone tissue engineering applications.
Junping Wang; Chandra M Valmikinathan; Wei Liu; Cato T Laurencin; Xiaojun Yu
Publication Detail:
Type:  Evaluation Studies; Journal Article    
Journal Detail:
Title:  Journal of biomedical materials research. Part A     Volume:  93     ISSN:  1552-4965     ISO Abbreviation:  J Biomed Mater Res A     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-03-25     Completed Date:  2010-06-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101234237     Medline TA:  J Biomed Mater Res A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  753-62     Citation Subset:  IM    
Copyright Information:
Copyright 2009 Wiley Periodicals, Inc.
Department of Chemical, Biomedical and Materials Engineering, Stevens Institute of Technology, Hoboken, New Jersey 07030, USA.
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MeSH Terms
Biocompatible Materials / chemistry
Bone Regeneration / physiology
Bone and Bones / physiology*
Cell Adhesion
Cell Differentiation
Cell Proliferation
Cells, Cultured
Compressive Strength
Electrochemical Techniques / methods
Materials Testing
Microscopy, Electron, Scanning
Nanofibers / chemistry*
Osteoblasts / cytology,  metabolism
Polyesters / chemistry
Surface Properties
Tissue Engineering* / instrumentation,  methods
Tissue Scaffolds / chemistry*
Reg. No./Substance:
0/Biocompatible Materials; 0/Polyesters; 24980-41-4/polycaprolactone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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