Document Detail

Spindle imaging: a marker for embryo development and implantation.
MedLine Citation:
PMID:  17727849     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To examine whether the presence of birefringent spindles in living human oocytes can be used as a predictive factor associated with embryo morphology to allow embryo selection before transfer and its association with IVF outcomes. DESIGN: Prospective study. SETTING: Assisted reproduction center in Brazil. PATIENT(S): One hundred fifty-seven patients undergoing intracytoplasmic sperm injection cycles, resulting in 1,097 metaphase II oocytes. INTERVENTION(S): Meiotic spindles were evaluated before intracytoplasmic sperm injection in all metaphase II oocytes. MAIN OUTCOME MEASURE(S): Meiotic spindles' imaging and fertilization rate, embryo development, and implantation rate. RESULT(S): Birefringent spindles were detected in 65.9% (SD group). The normal fertilization rate and rate of early-cleavaged embryos were higher in the SD group compared with in the spindle-non-detected (SND) group. When only embryos from the SD group were selected for transfer, the pregnancy and implantation rates were 44.4% and 23.0%, and when only embryos from the SND group were transferred, those rates were 18.2% and 8.7%, respectively (statistically significant differences). CONCLUSION(S): Spindle visualization can be an important tool for predicting better fertilization potential, embryo development, and clinical outcomes, suggesting that embryo selection for transfer may be based not only on embryo morphology but also on oocyte nuclear maturity.
Camila Madaschi; Tatiana Carvalho de Souza Bonetti; Daniela Paes de Almeida Ferreira Braga; Fabio Firmbach Pasqualotto; Assumpto Iaconelli; Edson Borges
Related Documents :
10502419 - Tamoxifen and endometrial pathologies: a prospective study.
9872489 - Patient noncompliance with hormone replacement therapy: a nationwide estimate using a l...
9138869 - Treatment of submucous fibroids, and outcome of assisted conception.
9782139 - Ultrasonographic prediction of the efficacy of gnrh agonist therapy before laparoscopic...
20723889 - Levonorgestrel-releasing intrauterine system (lng-ius) as an effective treatment option...
9583069 - Laparoscopic retroperitoneal hysteropexy. a randomized trial.
22938079 - Neurosurgical complications of left ventricular assist devices in children.
23539709 - The long-term clinical outcome after pelvic ring injuries.
20046519 - Comparison of azasetron and ondansetron for preventing postoperative nausea and vomitin...
Publication Detail:
Type:  Clinical Trial; Journal Article     Date:  2007-08-29
Journal Detail:
Title:  Fertility and sterility     Volume:  90     ISSN:  1556-5653     ISO Abbreviation:  Fertil. Steril.     Publication Date:  2008 Jul 
Date Detail:
Created Date:  2008-07-01     Completed Date:  2008-07-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372772     Medline TA:  Fertil Steril     Country:  United States    
Other Details:
Languages:  eng     Pagination:  194-8     Citation Subset:  IM    
Fertility-Assisted Fertilization Center, São Paulo, Brazil.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Embryo Implantation*
Embryo Transfer
Embryonic Development
Infertility / therapy*
Microscopy, Polarization
Mitotic Spindle Apparatus / ultrastructure*
Oocyte Retrieval*
Oocytes / growth & development,  ultrastructure*
Prospective Studies
Sperm Injections, Intracytoplasmic*
Treatment Outcome

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  The influence of sperm morphology on preimplantation genetic diagnosis cycles outcome.
Next Document:  Prolonged stimulation with tumor necrosis factor-alpha induced partial methylation at PR-B promoter ...