Document Detail


Spindle cell carcinoma of head and neck: an immunohistochemical and molecular approach to its pathogenesis.
MedLine Citation:
PMID:  16731596     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Spindle cell carcinoma (SpCC) is a rare microscopic type of cancer of the mouth and oropharynx. Although SpCC is thought to arise from squamous cell carcinoma (SCC), it carries a worse prognosis.
AIM: To find out the difference in immunohistochemical expression of cytokeratin, vimentin and smooth-muscle actin, and mutational alterations in the K-ras oncogene between the two tumours, in an attempt to characterise SpCC.
METHODS: Immunohistochemical analysis was performed by standard avidin-biotin complex method in 35 cases each of SpCCs and SCCs. DNA extracted from paraffin wax-embedded tumours was used for PCR followed by single-strand conformation polymorphism for mutational analysis of K-ras exon 1 and exon 2.
RESULTS: In the SpCC group, cytokeratin positivity was significantly higher in epithelial areas (52.2%) than in spindle cell areas (16.1%), whereas vimentin was more positive in spindle cell areas (18.7%) than epithelial areas (2.7%). Cells intermediate between epithelial and spindle cell areas were consistently positive for both cytokeratin and vimentin. Cytokeratin was found to be significantly more positive in SCC (72.6%) than the squamous component and spindle cell component of SpCC. In this study, no mutation was detected in the K-ras gene of either the SpCC or SCC group.
CONCLUSIONS: The spindle cell component of SpCC is intermixed with cells that are morphologically mesenchymal but express dual antigen-positivity characteristic of epithelial (cytokeratin) and mesenchymal (vimentin) cells. These, possibly, are cells in transition suggesting that SpCC may be a sarcomatous metaplasia of SCC.
Authors:
Ruchika Gupta; Sompal Singh; Suresh Hedau; Sonu Nigam; Bhudev C Das; Ishwar Singh; Ashish Kumar Mandal
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Publication Detail:
Type:  Journal Article     Date:  2006-05-26
Journal Detail:
Title:  Journal of clinical pathology     Volume:  60     ISSN:  0021-9746     ISO Abbreviation:  J. Clin. Pathol.     Publication Date:  2007 May 
Date Detail:
Created Date:  2007-05-21     Completed Date:  2007-06-28     Revised Date:  2013-06-07    
Medline Journal Info:
Nlm Unique ID:  0376601     Medline TA:  J Clin Pathol     Country:  England    
Other Details:
Languages:  eng     Pagination:  472-5     Citation Subset:  AIM; IM    
Affiliation:
Department of Pathology, Maulana Azad Medical College, New Delhi, India. rucihka257@yahoo.com
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MeSH Terms
Descriptor/Qualifier:
Actins / metabolism
Adult
Aged
Aged, 80 and over
Carcinoma, Squamous Cell / genetics,  metabolism*,  pathology
DNA Mutational Analysis / methods
DNA, Neoplasm / genetics
Female
Genes, ras
Genetic Predisposition to Disease
Head and Neck Neoplasms / genetics,  metabolism*,  pathology
Humans
Immunoenzyme Techniques
Keratins / metabolism
Male
Middle Aged
Neoplasm Proteins / metabolism*
Polymerase Chain Reaction / methods
Polymorphism, Single-Stranded Conformational
Prospective Studies
Vimentin / metabolism
Chemical
Reg. No./Substance:
0/Actins; 0/DNA, Neoplasm; 0/Neoplasm Proteins; 0/Vimentin; 68238-35-7/Keratins
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