Document Detail


Spinal cord injury-induced expression of TrkA, TrkB, phosphorylated CREB, and c-Jun in rat lumbosacral dorsal root ganglia.
MedLine Citation:
PMID:  15611995     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Previous studies have demonstrated increased expression and phosphorylation of tyrosine kinase receptor (TrkA, TrkB) in lumbosacral DRG after chronic (6 weeks) spinal cord (T8-T10) injury. This study examined the effects of acute SCI (48 hours, 2 weeks) on TrkA and TrkB expression and phosphorylation, and CREB and c-Jun expression in DRG. A significant increase in the number of TrkA- (1.5-3-fold; P < or = 0.05), TrkB- (1.3-2.0-fold; P < or = 0.05), and phosphorylated Trk (pTrk)-immunoreactive (1.5-3-fold; P < or = 0.05) cells was observed in the L1, L6, and S1 DRG 48 hours, 2, or 6 weeks after SCI. A significant increase in the number of phosphorylated (p-) CREB-immunoreactive cells was observed in the L1, L2, L6, and S1 DRG 48 hours, 2, or 6 weeks after SCI. The largest changes in p-CREB-immunoreactivity were in L1 and L2 DRG (10-fold; P <or= 0.01) at 48 hours after SCI; however, changes were modest in bladder afferent neurons. After SCI, the overall number of c-Jun-immunoreactive cells in L1, L2, and S1 DRG was dramatically increased (3-10-fold; P < or = 0.01); however, only a low percentage of bladder afferent cells expressed c-Jun-IR before or after SCI. In summary, these results suggest that TrkA or TrkB may be involved in reorganization of micturition pathways after SCI. However, CREB or c-Jun may not be downstream transcription factors in Trk-mediated signaling cascades in micturition reflex pathways after SCI but may play a role in other, nonbladder SCI-induced changes.
Authors:
Li-Ya Qiao; Margaret A Vizzard
Related Documents :
12441295 - Impaired intervertebral disc formation in the absence of jun.
8964085 - Prostaglandin e2 inhibits the interleukin-2 promoter activity through down-regulation o...
7592995 - Requirement of ap-1 for ceramide-induced apoptosis in human leukemia hl-60 cells.
1349165 - V-erba and c-erba proteins enhance transcriptional activation by c-jun.
12706485 - The egr-1 gene is induced by dna-damaging agents and non-genotoxic drugs in both normal...
20723665 - Effect of bovine lactoferricin on dna methyltransferase 1 levels in jurkat t-leukemia c...
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of comparative neurology     Volume:  482     ISSN:  0021-9967     ISO Abbreviation:  J. Comp. Neurol.     Publication Date:  2005 Feb 
Date Detail:
Created Date:  2004-12-27     Completed Date:  2005-04-18     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0406041     Medline TA:  J Comp Neurol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  142-54     Citation Subset:  IM    
Copyright Information:
2004 Wiley-Liss, Inc
Affiliation:
Department of Neurology, University of Vermont College of Medicine, Burlington, Vermont 05405, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Acute Disease
Animals
Cyclic AMP Response Element-Binding Protein / metabolism*
Female
Ganglia, Spinal / cytology,  metabolism*
Lumbosacral Region
Neural Pathways / cytology,  metabolism
Neurons, Afferent / metabolism*
Phosphorylation
Proto-Oncogene Proteins c-jun / metabolism*
Rats
Rats, Wistar
Receptor, trkA / metabolism*
Receptor, trkB / metabolism
Spinal Cord Injuries / metabolism*
Urinary Bladder / innervation
Urination / physiology
Grant Support
ID/Acronym/Agency:
DK051369/DK/NIDDK NIH HHS; DK060481/DK/NIDDK NIH HHS; DK065989/DK/NIDDK NIH HHS; NS040769/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Cyclic AMP Response Element-Binding Protein; 0/Proto-Oncogene Proteins c-jun; EC 2.7.10.1/Receptor, trkA; EC 2.7.10.1/Receptor, trkB

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Glycinergic neurons expressing enhanced green fluorescent protein in bacterial artificial chromosome...
Next Document:  Effect of neurokinins on canine prostate cell physiology.