Document Detail

Spinal cord injury-induced expression of TrkA, TrkB, phosphorylated CREB, and c-Jun in rat lumbosacral dorsal root ganglia.
MedLine Citation:
PMID:  15611995     Owner:  NLM     Status:  MEDLINE    
Previous studies have demonstrated increased expression and phosphorylation of tyrosine kinase receptor (TrkA, TrkB) in lumbosacral DRG after chronic (6 weeks) spinal cord (T8-T10) injury. This study examined the effects of acute SCI (48 hours, 2 weeks) on TrkA and TrkB expression and phosphorylation, and CREB and c-Jun expression in DRG. A significant increase in the number of TrkA- (1.5-3-fold; P < or = 0.05), TrkB- (1.3-2.0-fold; P < or = 0.05), and phosphorylated Trk (pTrk)-immunoreactive (1.5-3-fold; P < or = 0.05) cells was observed in the L1, L6, and S1 DRG 48 hours, 2, or 6 weeks after SCI. A significant increase in the number of phosphorylated (p-) CREB-immunoreactive cells was observed in the L1, L2, L6, and S1 DRG 48 hours, 2, or 6 weeks after SCI. The largest changes in p-CREB-immunoreactivity were in L1 and L2 DRG (10-fold; P <or= 0.01) at 48 hours after SCI; however, changes were modest in bladder afferent neurons. After SCI, the overall number of c-Jun-immunoreactive cells in L1, L2, and S1 DRG was dramatically increased (3-10-fold; P < or = 0.01); however, only a low percentage of bladder afferent cells expressed c-Jun-IR before or after SCI. In summary, these results suggest that TrkA or TrkB may be involved in reorganization of micturition pathways after SCI. However, CREB or c-Jun may not be downstream transcription factors in Trk-mediated signaling cascades in micturition reflex pathways after SCI but may play a role in other, nonbladder SCI-induced changes.
Li-Ya Qiao; Margaret A Vizzard
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of comparative neurology     Volume:  482     ISSN:  0021-9967     ISO Abbreviation:  J. Comp. Neurol.     Publication Date:  2005 Feb 
Date Detail:
Created Date:  2004-12-27     Completed Date:  2005-04-18     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0406041     Medline TA:  J Comp Neurol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  142-54     Citation Subset:  IM    
Copyright Information:
2004 Wiley-Liss, Inc
Department of Neurology, University of Vermont College of Medicine, Burlington, Vermont 05405, USA.
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MeSH Terms
Acute Disease
Cyclic AMP Response Element-Binding Protein / metabolism*
Ganglia, Spinal / cytology,  metabolism*
Lumbosacral Region
Neural Pathways / cytology,  metabolism
Neurons, Afferent / metabolism*
Proto-Oncogene Proteins c-jun / metabolism*
Rats, Wistar
Receptor, trkA / metabolism*
Receptor, trkB / metabolism
Spinal Cord Injuries / metabolism*
Urinary Bladder / innervation
Urination / physiology
Grant Support
Reg. No./Substance:
0/Cyclic AMP Response Element-Binding Protein; 0/Proto-Oncogene Proteins c-jun; EC, trkA; EC, trkB

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