Document Detail

Spinal cord injury-induced astrocyte migration and glial scar formation: effects of magnetic stimulation frequency.
MedLine Citation:
PMID:  21355419     Owner:  NLM     Status:  In-Process    
The effects of magnetic stimulation on spinal cord injury-induced migration of white matter astrocytes were studied using an established animal model. Ethidium bromide was injected into the dorsal spinal cord funiculus of adult Sprague-Dawley rats on the left side at T10-11. Animals then received 1.52 Tesla-pulsed magnetic stimulation for 5 min at different frequencies (0-20 Hz) for 14 consecutive days. Selected animals received the non-competitive MEK1/2 inhibitor U0126 (10 microM), prior to stimulation at 10 Hz. Lesion volumes were measured in hematoxylin/eosin-stained sections. Expression of glial fibrillary acidic protein (GFAP), microtubule associated protein-2 (MAP-2) and extra-cellular signal-regulated kinasel/2 (ERK1/2) near the epicenter of injury was examined by Western blotting with quantification using an image analysis system. Lesion volumes decreased and GFAP and p-ERK1/2 expression increased with increasing magnetic stimulation frequency (0-10 Hz). MAP-2 expression was not affected at any frequency. Pretreatment with U0126 reduced GFAP and ERK1/2 expression and increased lesion volumes in response to stimulation at 10 Hz. It is concluded that magnetic stimulation increases the migration of astrocytes to spinal cord lesions. Activation of the ERK1/2 signaling pathway is proposed to mediate astrocyte migration and glial scar formation in response to spinal cord injury.
Zhe Li; Zheng-Yu Fang; Liang Xiong; Xiao-Lin Huang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Indian journal of biochemistry & biophysics     Volume:  47     ISSN:  0301-1208     ISO Abbreviation:  Indian J. Biochem. Biophys.     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2011-03-01     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0310774     Medline TA:  Indian J Biochem Biophys     Country:  India    
Other Details:
Languages:  eng     Pagination:  359-63     Citation Subset:  IM    
Department of Rehabilitation Medicine, WHO Collaborating Centre for Training and Research in Rehabilitaion (CHN-60) Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
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