Document Detail


Spin-trapping and direct EPR investigations on the hepatotoxic and hepatocarcinogenic actions of luteoskyrin, an anthraquinoid mycotoxin produced by Penicillium islandicum Sopp. Generations of superoxide anion and luteoskyrin semiquinone radical in the redox systems consisted of luteoskyrin and liver NADPH- or NADH-dependent reductases.
MedLine Citation:
PMID:  7647918     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Luteoskyrin is a hepatotoxic and hepatocarcinogenic bisdihydroanthraquinone produced by Penicillium islandicum Sopp. By observing the EPR spectra of DMPO-spin adducts and luteoskyrin semiquinone radical, we investigated in vitro whether luteoskyrin is reduced to its semiquinone radical leading to the generation of active oxygen species in redox systems catalyzed by NADPH-dependent cytochrome reductases of the liver. We found (1) the formation of luteoskyrin semiquinone radical in the NADPH-cytochrome P-450 reductase system under anaerobic conditions, (2) the generation of O2- in the systems composed of luteoskyrin, NAD(P)H, and either rat liver microsomal NADPH-cytochrome P-450 reductases or submitochondrial particles and (3) dicoumarol showed no effect on the O2- generation in the case of submitochondrial particles. From these results we proposed that luteoskyrin liver injuries are induced by the active oxygen species generated in the process of autoxidation of luteoskyrin semiquinone radical which is produced in the one-electron redox systems catalyzed by the liver NAD(P)H-dependent cytochrome reductases.
Authors:
I Ueno; M Sekijima; M Hoshino; H Ohya-Nishiguchi; Y Ueno
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Free radical research     Volume:  23     ISSN:  1071-5762     ISO Abbreviation:  Free Radic. Res.     Publication Date:  1995 Jul 
Date Detail:
Created Date:  1995-09-26     Completed Date:  1995-09-26     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  9423872     Medline TA:  Free Radic Res     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  41-50     Citation Subset:  IM    
Affiliation:
Institute of Physical and Chemical Research, Saitama, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Carcinogenicity Tests
Drug-Induced Liver Injury / enzymology,  etiology*
Electron Spin Resonance Spectroscopy
Free Radicals
Liver / enzymology
Liver Neoplasms / chemically induced*,  enzymology
Mycotoxins / toxicity*
NADH, NADPH Oxidoreductases / metabolism
Naphthoquinones / toxicity*
Oxidation-Reduction
Penicillium / metabolism*
Rats
Rats, Sprague-Dawley
Superoxides / metabolism*
Chemical
Reg. No./Substance:
0/Free Radicals; 0/Mycotoxins; 0/Naphthoquinones; 11062-77-4/Superoxides; 21884-44-6/luteoskyrin; EC 1.6.-/NADH, NADPH Oxidoreductases

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