Document Detail


Spin-labeled psoralen probes for the study of DNA dynamics.
MedLine Citation:
PMID:  7578089     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Six nitroxide spin-labeled psoralen derivative have been synthesized and evaluated as probes for structural and dynamic studies. Sequence specific photoaddition of these derivatives to DNA oligonucleotides resulted in site-specifically cross-linked and spin-labeled oligomers. Comparison of the general line shape features of the observed electron paramagnetic resonance (EPR) spectra of several duplexes ranging in size from 8 to 46 base pairs with simulated EPR spectra indicate that the nitroxide spin-label probe reports the global tumbling motion of the oligomers. While there is no apparent large amplitude motion of the psoralen other than the overall tumbling of the DNA on the time scales investigated, there are some indications of bending and other residual motions. The (A)BC excinuclease DNA repair system detects structural or dynamic features of the DNA that distinguish between damaged and undamaged DNA and are independent of the intrinsic structure of the lesion. NMR studies have shown that psoralen-cross-linked DNA has altered backbone dynamics and conformational populations in the immediate vicinity of the adduct [Emsley et al. (1993) J. Am. Chem. Soc. 115, 7765-7771; Spielmann et al. (1995) Proc. Natl. Acad. Sci. U.S.A. 92, 2345-2349]. We suggested that the signal for recognition of a lesion to be repaired is in the sugar--phosphate backbone and not in the damaged base(s).
Authors:
H P Spielmann; D Y Chi; N G Hunt; M P Klein; J E Hearst
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochemistry     Volume:  34     ISSN:  0006-2960     ISO Abbreviation:  Biochemistry     Publication Date:  1995 Nov 
Date Detail:
Created Date:  1995-12-18     Completed Date:  1995-12-18     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370623     Medline TA:  Biochemistry     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  14801-14     Citation Subset:  IM    
Affiliation:
Structural Biology Division, Lawrence Berkeley Laboratory, University of California, Berkeley 94720, USA.
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
Computer Graphics
Cross-Linking Reagents
DNA / chemistry*
DNA Damage*
DNA Repair
Electron Spin Resonance Spectroscopy
Electrophoresis, Polyacrylamide Gel
Magnetic Resonance Spectroscopy
Models, Molecular
Molecular Probes / chemistry
Molecular Sequence Data
Molecular Structure
Nucleic Acid Conformation*
Photolysis
Psoralens* / chemical synthesis
Spin Labels* / chemical synthesis
Grant Support
ID/Acronym/Agency:
GM-14966/GM/NIGMS NIH HHS; GM-41911/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Cross-Linking Reagents; 0/Molecular Probes; 0/Psoralens; 0/Spin Labels; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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