| Spin-label studies of the sulfhydryl environment in bovine plasma albumin. 1. The N--F transition and acid expansion. | |
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MedLine Citation:
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PMID: 26387 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The environment of the sulfhydryl group in plasma albumin was previously characterized by employing spin-labels of varying chain lengths (Hull, H. H., Chang, R., & Kaplan, L. J. (1975) Biochim. Biophys. Acta 400, 132). It was established that the sulfhydryl is in a crevice approximately 10 A deep but this crevice was not identified further. We now report the results of titrating albumin through the acidic conformational transitions while monitoring the electron-spin resonance of the bound nitroxide. With short spin-labels a general change is observed as the pH is lowered but the N--F transition is not discernible. However, with a spin label previously shown to project to the lip on the crevice a clear N--F transition as well as the subsequent acid expansion are observed. These results indicate that the sulfhydryl is in the crevice, formed by the domains of albumin, which opens during the N--F transition. Further results indicate that bound fatty acids do not influence the integrity of the sulfhydryl environment at neutral pH. |
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Authors:
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C N Cornell; L J Kaplan |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biochemistry Volume: 17 ISSN: 0006-2960 ISO Abbreviation: Biochemistry Publication Date: 1978 May |
Date Detail:
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Created Date: 1978-08-28 Completed Date: 1978-08-28 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0370623 Medline TA: Biochemistry Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1750-4 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Chemical Phenomena Chemistry Cyclic N-Oxides Electron Spin Resonance Spectroscopy Hydrogen-Ion Concentration Maleimides Protein Conformation Serum Albumin, Bovine* Spin Labels Sulfhydryl Compounds |
| Chemical | |
Reg. No./Substance:
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0/Cyclic N-Oxides; 0/Maleimides; 0/Serum Albumin, Bovine; 0/Spin Labels; 0/Sulfhydryl Compounds |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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