Document Detail


Spiking Survanta with synthetic surfactant peptides improves oxygenation in surfactant-deficient rats.
MedLine Citation:
PMID:  9310004     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The hypothesis that the in vivo function of Survanta (Beractant) can be improved by supplementation with synthetic surfactant peptides B and C was tested in a surfactant-deficient rat model. Full length surfactant protein-B (SP-B1-78) (B) and palmitoylated surfactant protein-C (SP-C1-35) (C), and synthetic KL4 peptide were added to Survanta after extraction, creating extracted Survanta (ES) with 1% B, 2% B, and 2% B plus 1% C, or mixed with Survanta without extraction, creating modified Survanta (S) with 2% B, 2% B plus 1% C, and 2% KL4. Adult rats were ventilated with 100% oxygen, tidal volumes (VT) of 7.5 ml/kg and a rate of 60/min, and were lavaged until the PaO2 dropped below 80 mm Hg, when 100 mg/kg of surfactant was instilled. After 15 to 60 min of ventilation, pressure-volume (P-V) curves were generated in situ. Instillation of ES or S with 2% B plus 1% C led to the greatest increase in oxygenation, closely followed by ES and S with 2% B, and more distantly by S plus 2% KL4. TLC was comparable among the ES and S groups, but greater than that of air-placebo controls. These data suggest that spiking of Survanta with synthetic SP-B and SP-C increased oxygenation more effectively than B or KL4 alone in this surfactant-deficient rat model.
Authors:
F J Walther; J Hernández-Juviel; R Bruni; A J Waring
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  American journal of respiratory and critical care medicine     Volume:  156     ISSN:  1073-449X     ISO Abbreviation:  Am. J. Respir. Crit. Care Med.     Publication Date:  1997 Sep 
Date Detail:
Created Date:  1997-10-28     Completed Date:  1997-10-28     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9421642     Medline TA:  Am J Respir Crit Care Med     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  855-61     Citation Subset:  AIM; IM    
Affiliation:
Department of Pediatrics, Charles R. Drew University of Medicine and Science, Los Angeles, California, USA.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Biological Products*
Blood Gas Analysis
Disease Models, Animal
Drug Combinations
Drug Evaluation, Preclinical
Humans
Hyaline Membrane Disease / drug therapy*,  metabolism
Infant, Newborn
Instillation, Drug
Male
Molecular Sequence Data
Oxygen / blood*
Proteolipids / chemistry,  therapeutic use*
Pulmonary Surfactants / chemistry,  therapeutic use*
Rats
Rats, Sprague-Dawley
Grant Support
ID/Acronym/Agency:
HL55534/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Biological Products; 0/Drug Combinations; 0/Proteolipids; 0/Pulmonary Surfactants; 108778-82-1/beractant; 7782-44-7/Oxygen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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