| Sphingosine-1-phosphate regulates the expression of adherens junction protein e-cadherin and enhances intestinal epithelial cell barrier function. | |
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MedLine Citation:
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PMID: 20936358 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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BACKGROUND: The regulation of intestinal barrier permeability is important in the maintenance of normal intestinal physiology. Sphingosine-1-phosphate (S1P) has been shown to play a pivotal role in enhancing barrier function in several non-intestinal tissues. The current study determined whether S1P regulated function of the intestinal epithelial barrier by altering expression of E-cadherin, an important protein in adherens junctions. METHODS: Studies were performed upon cultured differentiated IECs (IEC-Cdx2L1 line) using standard techniques. RESULTS: S1P treatment significantly increased levels of E-cadherin protein and mRNA in intestinal epithelial cells (IECs) and also led to E-cadherin localizing strongly to the cell-cell border. S1P also improved the barrier function as indicated by a decrease in 14C-mannitol paracellular permeability and an increase in transepithelial electrical resistance (TEER) in vitro. CONCLUSIONS: These results indicate that S1P increases levels of E-cadherin, both in cellular amounts and at the cell-cell junctions, and leads to improved barrier integrity in cultured intestinal epithelial cells. |
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Authors:
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Jose Greenspon; Ruiyun Li; Lan Xiao; Jaladanki N Rao; Rex Sun; Eric D Strauch; Terez Shea-Donohue; Jian-Ying Wang; Douglas J Turner |
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Publication Detail:
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Type: Journal Article Date: 2010-10-09 |
Journal Detail:
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Title: Digestive diseases and sciences Volume: 56 ISSN: 1573-2568 ISO Abbreviation: Dig. Dis. Sci. Publication Date: 2011 May |
Date Detail:
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Created Date: 2011-04-26 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7902782 Medline TA: Dig Dis Sci Country: United States |
Other Details:
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Languages: eng Pagination: 1342-53 Citation Subset: AIM; IM |
Affiliation:
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Cell Biology Group, Department of Surgery, University of Maryland School of Medicine, Baltimore, MD, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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