Document Detail


Sphingosine-1-phosphate regulates the expression of adherens junction protein E-cadherin and enhances intestinal epithelial cell barrier function.
MedLine Citation:
PMID:  20936358     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: The regulation of intestinal barrier permeability is important in the maintenance of normal intestinal physiology. Sphingosine-1-phosphate (S1P) has been shown to play a pivotal role in enhancing barrier function in several non-intestinal tissues. The current study determined whether S1P regulated function of the intestinal epithelial barrier by altering expression of E-cadherin, an important protein in adherens junctions.
METHODS: Studies were performed upon cultured differentiated IECs (IEC-Cdx2L1 line) using standard techniques.
RESULTS: S1P treatment significantly increased levels of E-cadherin protein and mRNA in intestinal epithelial cells (IECs) and also led to E-cadherin localizing strongly to the cell-cell border. S1P also improved the barrier function as indicated by a decrease in 14C-mannitol paracellular permeability and an increase in transepithelial electrical resistance (TEER) in vitro.
CONCLUSIONS: These results indicate that S1P increases levels of E-cadherin, both in cellular amounts and at the cell-cell junctions, and leads to improved barrier integrity in cultured intestinal epithelial cells.
Authors:
Jose Greenspon; Ruiyun Li; Lan Xiao; Jaladanki N Rao; Rex Sun; Eric D Strauch; Terez Shea-Donohue; Jian-Ying Wang; Douglas J Turner
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2010-10-09
Journal Detail:
Title:  Digestive diseases and sciences     Volume:  56     ISSN:  1573-2568     ISO Abbreviation:  Dig. Dis. Sci.     Publication Date:  2011 May 
Date Detail:
Created Date:  2011-04-26     Completed Date:  2011-07-07     Revised Date:  2014-08-24    
Medline Journal Info:
Nlm Unique ID:  7902782     Medline TA:  Dig Dis Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1342-53     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
Adherens Junctions / metabolism*
Animals
Cadherins / genetics,  metabolism*
Calcium / metabolism
Cell Adhesion Molecules / genetics,  metabolism
Cells, Cultured
Epithelial Cells / drug effects,  physiology*
Gene Expression Regulation / drug effects,  physiology
Intestinal Mucosa / cytology*
Lysophospholipids / pharmacology*
RNA Interference
Sphingosine / analogs & derivatives*,  pharmacology
beta Catenin
Grant Support
ID/Acronym/Agency:
R01 AI049316/AI/NIAID NIH HHS; T32 DK067872/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Cadherins; 0/Cell Adhesion Molecules; 0/Lysophospholipids; 0/beta Catenin; 26993-30-6/sphingosine 1-phosphate; NGZ37HRE42/Sphingosine; SY7Q814VUP/Calcium
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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