| Spermine signalling in tobacco: activation of mitogen-activated protein kinases by spermine is mediated through mitochondrial dysfunction. | |
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MedLine Citation:
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PMID: 14675447 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Polyamines (PAs) play important roles in cell proliferation, growth and environmental stress responses of all living organisms. In this study, we examine whether these compounds act as signal mediators. Spermine (Spm) specifically activated protein kinases of tobacco leaves, which were identified as salicylic acid (SA)-induced protein kinase (SIPK) and wound-induced protein kinase (WIPK), using specific antibodies. Upon Spm treatment, upregulation of WIPK, but not SIPK, was observed. Spm-induced mitogen-activated protein kinases (MAPKs) activation and WIPK upregulation were prevented upon pre-treatment with antioxidants and Ca2+ channel blockers. Additionally, Spm specifically stimulated expression of the alternative oxidase (AOX) gene, which was disrupted by these antioxidants and Ca2+ channel blockers. Bongkrekic acid (BK), an inhibitor of the opening of mitochondrial permeability transition (PT) pores, suppressed MAPKs activation and accumulation of WIPK and AOX mRNA. Our data collectively suggest that Spm causes mitochondrial dysfunction via a signalling pathway in which reactive oxygen species and Ca2+ influx are involved. As a result, the phosphorylation activities of the two MAPK enzymes SIPK and WIPK are stimulated. |
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Authors:
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Yoshihiro Takahashi; Thomas Berberich; Atsushi Miyazaki; Shigemi Seo; Yuko Ohashi; Tomonobu Kusano |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Plant journal : for cell and molecular biology Volume: 36 ISSN: 0960-7412 ISO Abbreviation: Plant J. Publication Date: 2003 Dec |
Date Detail:
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Created Date: 2003-12-16 Completed Date: 2004-04-09 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 9207397 Medline TA: Plant J Country: England |
Other Details:
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Languages: eng Pagination: 820-9 Citation Subset: IM |
Affiliation:
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Graduate School of Life Sciences, Tohoku University, 2-1-1 Katahira, Aoba, Sendai, Miyagi 980-8577, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Carbonyl Cyanide m-Chlorophenyl Hydrazone
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pharmacology Dimethyl Sulfoxide / pharmacology Enzyme Activation / drug effects Gene Expression Regulation, Enzymologic Gene Expression Regulation, Plant / genetics Kinetics Mitochondria / drug effects, enzymology*, physiology Mitogen-Activated Protein Kinases / metabolism* Molecular Weight Oxidoreductases / genetics Plant Leaves / enzymology Plants, Genetically Modified / genetics Signal Transduction / physiology* Spermine / pharmacology*, physiology* Tobacco / enzymology*, genetics |
| Chemical | |
Reg. No./Substance:
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555-60-2/Carbonyl Cyanide m-Chlorophenyl Hydrazone; 67-68-5/Dimethyl Sulfoxide; 71-44-3/Spermine; EC 1.-/Oxidoreductases; EC 1.-/alternative oxidase; EC 2.7.11.24/Mitogen-Activated Protein Kinases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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