Document Detail


Spermine signaling plays a significant role in the defense response of Arabidopsis thaliana to cucumber mosaic virus.
MedLine Citation:
PMID:  18922600     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have proposed that the polyamine spermine (Spm) functions as a signaling molecule to evoke defense reactions/cell death in avirulent pathogen-attacked tobacco plants. To understand its molecular basis in depth, Spm-responsive genes in Arabidopsis thaliana were identified by SuperSAGE analysis. Close to 90% of the Spm-responsive genes also responded during cucumber mosaic virus (CMV)-elicited hypersensitive response. Spm modulated the expression of genes of redox components, and genes involved in protein folding and secretion, protein degradation and defense. Two other prominent changes, the coordinately enhanced expression of members of the photorespiration pathway and a diversion in electron flow from the primary electron transfer chain of respiration to an alternative oxidase pathway, occurred in response to Spm. Spm activated the expression of 6 transcription factor genes including ZAT7, ZAT12, AtWRKY40 and AtbZIP60, of which the former three genes' products are currently assigned as components of H(2)O(2) signaling pathway, suggesting the involvement of H(2)O(2) in Spm-triggered responses. Since AtbZIP60 plays a proven master role in the unfolded protein response in Arabidopsis thaliana, it may function to control the expression of genes participating in protein folding and secretion, which were mentioned above. Spm induction and CMV-triggered up-regulation of the genes described mainly coincided and their induction was suppressed by inhibitors of Spm oxidation. Furthermore, treatment with those inhibitors prior to CMV inoculation allowed higher viral multiplication in Arabidopsis thaliana plants. These results support the existence of a Spm-signaling pathway in Arabidopsis thaliana and its significant role in defense against CMV.
Authors:
Yoshiko Mitsuya; Yoshihiro Takahashi; Thomas Berberich; Atsushi Miyazaki; Hideo Matsumura; Hideki Takahashi; Ryohei Terauchi; Tomonobu Kusano
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-10-14
Journal Detail:
Title:  Journal of plant physiology     Volume:  166     ISSN:  1618-1328     ISO Abbreviation:  J. Plant Physiol.     Publication Date:  2009 Apr 
Date Detail:
Created Date:  2009-03-17     Completed Date:  2009-06-22     Revised Date:  2009-10-01    
Medline Journal Info:
Nlm Unique ID:  9882059     Medline TA:  J Plant Physiol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  626-43     Citation Subset:  IM    
Affiliation:
Graduate School of Life Sciences, Tohoku University, Sendai, Miyagi, Japan.
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MeSH Terms
Descriptor/Qualifier:
Arabidopsis / drug effects,  genetics,  immunology*,  virology*
Base Sequence
Cell Respiration / drug effects
Cucumovirus / physiology*
Enzyme Inhibitors / pharmacology
Gene Expression Profiling
Gene Expression Regulation, Plant / drug effects
Genes, Plant
Immunity, Innate / drug effects,  genetics
Light
Molecular Sequence Data
Plant Diseases / genetics,  immunology,  virology
Plant Leaves / drug effects,  genetics
Signal Transduction / drug effects*
Spermine / pharmacology*
Transcription Factors / genetics,  metabolism
Up-Regulation / drug effects
Virus Replication / drug effects
Chemical
Reg. No./Substance:
0/Enzyme Inhibitors; 0/Transcription Factors; 71-44-3/Spermine
Comments/Corrections
Comment In:
Plant Signal Behav. 2009 Apr;4(4):316-8   [PMID:  19794850 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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