Document Detail


The spermatogonial stem cell niche in the collared peccary (Tayassu tajacu).
MedLine Citation:
PMID:  22262689     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In the seminiferous epithelium, spermatogonial stem cells (SSCs) are located in a particular environment called the "niche" that is controlled by the basement membrane, key testis somatic cells, and factors originating from the vascular network. However, the role of Leydig cells (LCs) as a niche component is not yet clearly elucidated. Recent studies showed that peccaries (Tayassu tajacu) present a peculiar LC cytoarchitecture in which these cells are located around the seminiferous tubule lobes, making the peccary a unique model for investigating the SSC niche. This peculiarity allowed us to subdivide the seminiferous tubule cross-sections in three different testis parenchyma regions (tubule-tubule, tubule-interstitium, and tubule-LC contact). Our aims were to characterize the different spermatogonial cell types and to determine the location and/or distribution of the SSCs along the seminiferous tubules. Compared to differentiating spermatogonia, undifferentiated spermatogonia (A(und)) presented a noticeably higher nuclear volume (P < 0.05), allowing an accurate evaluation of their distribution. Immunostaining analysis demonstrated that approximately 93% of A(und) were GDNF receptor alpha 1 positive (GFRA1(+)), and these cells were preferentially located adjacent to the interstitial compartment without LCs (P < 0.05). The expression of colony-stimulating factor 1 was observed in LCs and peritubular myoid cells (PMCs), whereas its receptor was present in LCs and in GFRA1(+) A(und). Taken together, our findings strongly suggest that LCs, different from PMCs, might play a minor role in the SSC niche and physiology and that these steroidogenic cells are probably involved in the differentiation of A(und) toward type A(1) spermatogonia.
Authors:
Paulo Henrique A Campos-Junior; Guilherme M J Costa; Samyra M S N Lacerda; José V Rezende-Neto; Ana M de Paula; Marie-Claude Hofmann; Luiz R de França
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-05-17
Journal Detail:
Title:  Biology of reproduction     Volume:  86     ISSN:  1529-7268     ISO Abbreviation:  Biol. Reprod.     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-05-18     Completed Date:  2012-09-17     Revised Date:  2013-06-26    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  United States    
Other Details:
Languages:  eng     Pagination:  155, 1-10     Citation Subset:  IM    
Affiliation:
Laboratory of Cellular Biology, Department of Morphology, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
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MeSH Terms
Descriptor/Qualifier:
Animals
Artiodactyla / physiology
Glial Cell Line-Derived Neurotrophic Factor Receptors / analysis
Leydig Cells / cytology,  metabolism
Macrophage Colony-Stimulating Factor / biosynthesis
Male
Receptor, Macrophage Colony-Stimulating Factor / analysis
Seminiferous Tubules / cytology
Spermatogenesis / physiology
Spermatogonia / cytology,  metabolism*
Stem Cell Niche / physiology*
Stem Cells / cytology,  metabolism
Grant Support
ID/Acronym/Agency:
K02 HD054607/HD/NICHD NIH HHS; R01 HD044543/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Glial Cell Line-Derived Neurotrophic Factor Receptors; 81627-83-0/Macrophage Colony-Stimulating Factor; EC 2.7.10.1/Receptor, Macrophage Colony-Stimulating Factor
Comments/Corrections

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