Document Detail

Spermatogenic onset. II. FSH modulates mitotic activity of germ and Sertoli cells in immature rats.
MedLine Citation:
PMID:  3137178     Owner:  NLM     Status:  MEDLINE    
By using high doses of testosterone propionate (TP) endogenous FSH was lowered to non-detectable levels in immature rats of different ages. Combined administration of TP and human menopausal gonadotrophin (hMG) or purified human FSH (hFSH) restored circulating FSH to normal or supranormal levels. This experimental model was used to investigate the influences of hormones on the proliferation of Sertoli and germ cells. Mitoses in seminiferous tubules were counted after being blocked by administration of colchicine. The mitotic index so determined showed a reduction with FSH withdrawal and a significant increase when the hormone levels were restored. Testicular DNA content was determined in testicular homogenates and showed similar changes. Autoradiographs were prepared and 3H-thymidine incorporation into germ and Sertoli cells was quantified. It was found that hFSH induced a significant increase in the labelling indices of gonocytes, type A-spermatogonia and Sertoli cells. All of these changes were effective in rats younger than 10 days but no modifications in any of the parameters under study were observed after 20 days. It is concluded that FSH exerts a stimulatory effect on the proliferation of spermatogonia, Sertoli cells and testicular DNA content during the first 10 days of life.
I Almirón; H Chemes
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  International journal of andrology     Volume:  11     ISSN:  0105-6263     ISO Abbreviation:  Int. J. Androl.     Publication Date:  1988 Jun 
Date Detail:
Created Date:  1988-10-03     Completed Date:  1988-10-03     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8000141     Medline TA:  Int J Androl     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  235-46     Citation Subset:  IM    
Laboratory of Testicular Physiology and Pathology, Buenos Aires Children's Hospital, Buenos, Argentina.
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MeSH Terms
Colchicine / pharmacology
DNA / biosynthesis
Follicle Stimulating Hormone / blood*,  pharmacology
Menotropins / pharmacology
Mitotic Index
Sertoli Cells / cytology*
Sexual Maturation
Spermatozoa / cytology*
Testosterone / pharmacology
Reg. No./Substance:
58-22-0/Testosterone; 61489-71-2/Menotropins; 64-86-8/Colchicine; 9002-68-0/Follicle Stimulating Hormone; 9007-49-2/DNA

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