Document Detail


Spermatogenetic but not immunological defects in mice lacking the τCstF-64 polyadenylation protein.
MedLine Citation:
PMID:  21489638     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Alternative polyadenylation controls expression of genes in many tissues including immune cells and male germ cells. The τCstF-64 polyadenylation protein is expressed in both cell types, and we previously showed that Cstf2t, the gene encoding τCstF-64 was necessary for spermatogenesis and fertilization. Here we examine consequences of τCstF-64 loss in both germ cells and immune cells. Spermatozoa from Cstf2t null mutant (Cstf2t(-/-)) mice of ages ranging from 60 to 108 days postpartum exhibited severe defects in motility and morphology that were correlated with a decrease in numbers of round spermatids. Spermatozoa in these mice also displayed severe morphological defects at every age, especially in the head and midpiece. In the testicular epithelium, we saw normal numbers of cells in earlier stages of spermatogenesis, but reduced numbers of round spermatids in Cstf2t(-/-) mice. Although Leydig cell numbers were normal, we did observe reduced levels of plasma testosterone in the knockout animals, suggesting that reduced androgen might also be contributing to the Cstf2t(-/-) phenotype. Finally, while τCstF-64 was expressed in a variety of immune cell types in wild type mice, we did not find differences in secreted IgG or IgM or changes in immune cell populations in Cstf2t(-/-) mice, suggesting that τCstF-64 function in immune cells is either redundant or vestigial. Together, these data show that τCstF-64 function is primarily to support spermatogenesis, but only incidentally to support immune cell function.
Authors:
Kathy Jo Hockert; Kathleen Martincic; S M L C Mendis-Handagama; Lisa Ann Borghesi; Christine Milcarek; Brinda Dass; Clinton C MacDonald
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-04-13
Journal Detail:
Title:  Journal of reproductive immunology     Volume:  89     ISSN:  1872-7603     ISO Abbreviation:  J. Reprod. Immunol.     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-04-25     Completed Date:  2011-08-29     Revised Date:  2013-12-09    
Medline Journal Info:
Nlm Unique ID:  8001906     Medline TA:  J Reprod Immunol     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  26-37     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Movement / genetics
Cells, Cultured
Fertilization / genetics
Humans
Immune System / metabolism*,  pathology
Immunoglobulins / blood
Infertility, Male / genetics*,  metabolism,  pathology,  physiopathology
Male
Mice
Mice, Inbred C57BL
Mice, Knockout
Polyadenylation / genetics
RNA-Binding Proteins / genetics,  metabolism*
Spermatids / metabolism*,  pathology
Spermatogenesis / genetics
Spermatozoa / metabolism*,  pathology
Testosterone / blood
Grant Support
ID/Acronym/Agency:
HD037109/HD/NICHD NIH HHS; R01 HD037109/HD/NICHD NIH HHS; R01 HD037109-08/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/CSTF2T protein, human; 0/Immunoglobulins; 0/RNA-Binding Proteins; 3XMK78S47O/Testosterone
Comments/Corrections

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