| Sperm ubiquitination in patients with dysplasia of the fibrous sheath. | |
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MedLine Citation:
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PMID: 12151447 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Human sperm with structural abnormalities display an increased content of the cellular proteolytic marker peptide, ubiquitin. We investigated whether dysplasia of the fibrous sheath (DFS), a severe structural anomaly found in the sperm of some asthenozoospermic patients, is accompanied by (i) increased ubiquitination of the sperm surface and (ii) by increased ubiquitination of the sperm mitochondria. METHODS AND RESULTS: Five DFS patients and eight fertile donors were studied by immunocytochemistry with anti-ubiquitin antibodies. Increased cross-reactivity of the ubiquitinated mitochondrial epitopes was seen in 32-50% of DFS sperm, but only 2-4.1% of sperm from fertile donors. Sperm surface ubiquitination assessed by sperm-ubiquitin tag immunoassay (SUTI) and immunofluorescence demonstrated an increased sperm ubiquitination in all DFS patients. The average median value of ubiquitin-induced fluorescence in DFS patients was 25.8 counts (range 19.8-37.9), as opposed to 13.4 counts range (9.3-16.6) in fertile men. Sperm with 'stump tails', coiled tails, twin and triplet sperm, and clusters of immature spermatogenic cells were common. CONCLUSIONS: DFS sperm have increased cross-reactivity to anti-ubiquitin antibodies, a finding consistent with the ubiquitination of defective sperm shown in animal models. These results justify the use of ubiquitin-based assays for objective semen analysis in infertile men with heritable defects. |
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Authors:
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V Y Rawe; S Brugo Olmedo; A Benmusa; S M Shiigi; H E Chemes; P Sutovsky |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Human reproduction (Oxford, England) Volume: 17 ISSN: 0268-1161 ISO Abbreviation: Hum. Reprod. Publication Date: 2002 Aug |
Date Detail:
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Created Date: 2002-08-01 Completed Date: 2003-01-24 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8701199 Medline TA: Hum Reprod Country: England |
Other Details:
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Languages: eng Pagination: 2119-27 Citation Subset: IM |
Affiliation:
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Centro de Estudios en Ginecología y Reproducción, CEGyR, 1055-Buenos Aires, Argentina. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Membrane
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metabolism Congenital Abnormalities / metabolism Flow Cytometry Humans Immunoassay Infertility, Male / etiology* Male Microscopy, Electron Mitochondria / metabolism, ultrastructure Spermatozoa / abnormalities*, metabolism*, ultrastructure Ubiquitin / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Ubiquitin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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