Document Detail

Sperm morphology using strict criteria after Percoll density separation: influence on cleavage and pregnancy rates after in-vitro fertilization.
MedLine Citation:
PMID:  8582979     Owner:  NLM     Status:  MEDLINE    
The main purpose of the study was to evaluate the use of sperm morphology assessment by strict criteria on the post-Percoll separated spermatozoa used for oocyte insemination in an in-vitro fertilization programme. This study included a consecutive unselected series of 213 oocyte aspirations in 159 women. In 177 aspirations the patient had tubal infertility and in 36 unexplained infertility. Data have been analysed from 197 aspirations where the semen sample used for insemination had a normal sperm concentration (> or = 20 x 10(6)/ml). A total of 1413 oocytes were aspirated, resulting in 863 oocytes which were fertilized and cleaved (cleavage rate 61%). In all, 492 pre-embryos were transferred in 193 cycles, resulting in a pregnancy rate of 42% per transfer. Sperm morphology evaluation using strict criteria showed that Percoll separation significantly increased the percentage of sperm cells with normal morphology from 7.7 to 11.3%. Sperm morphology analysis showed that Percoll separation decreased the number of sperm samples in the 'poor prognosis pattern' group from 31 to 13% and increased the number of sperm samples classified as 'normal' from 16 to 33%. After Percoll separation the poor prognosis pattern group had a cleavage rate of 46%, which was significantly lower than in the good prognosis pattern and the normal groups. However, the poor prognosis pattern group had a significantly higher pregnancy rate than the normal group (P < 0.02).(ABSTRACT TRUNCATED AT 250 WORDS)
Z Yue; F J Meng; N Jørgensen; S Ziebe; A Nyboe Andersen
Related Documents :
14761839 - Effect of treating antibody-coated sperm with chymotrypsin on pregnancy rates following...
12084229 - Predictors of success in microsurgical correction of vasal and epididymal obstruction.
364049 - Sperm penetration in vitro of pig follicular oocytes matured in culture.
17689209 - Early pregnancy loss in sows after low dose, deep uterine artificial insemination with ...
8825169 - The impact of the total motile sperm count on the success of intrauterine insemination ...
8122939 - Effect of shortened exposure time to the critical period for ice crystal formation on s...
11579889 - The safety of the combination artesunate and pyrimethamine-sulfadoxine given during pre...
9363709 - Fertility after laparoscopic myomectomy: preliminary results.
10821959 - Suboptimal reproductive performance of dairy cattle kept in smallholder herds in a rura...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Human reproduction (Oxford, England)     Volume:  10     ISSN:  0268-1161     ISO Abbreviation:  Hum. Reprod.     Publication Date:  1995 Jul 
Date Detail:
Created Date:  1996-03-19     Completed Date:  1996-03-19     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8701199     Medline TA:  Hum Reprod     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1781-5     Citation Subset:  IM    
Department of Obstetrics and Gynecology, Herlev University Hospital, Denmark.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cell Separation / methods*
Cleavage Stage, Ovum*
Fertilization in Vitro*
Pregnancy Rate*
Spermatozoa / cytology*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Results of 55 intracytoplasmic sperm injection cycles in the treatment of male-immunological inferti...
Next Document:  Effects of nitric oxide on human spermatozoa: evidence that nitric oxide decreases sperm motility an...