Document Detail


Sperm membrane incorporation into oolemma contributes to the oolemma block to sperm penetration: evidence based on intracytoplasmic sperm injection experiments in the mouse.
MedLine Citation:
PMID:  9115725     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Mouse oocytes were fertilized by intracytoplasmic sperm injection (ICSI) and reinseminated after the removal of zonae pellucidae at pronuclear stage or at the 2-cell stage. Although these oocytes were activated normally by ICSI, as evidenced by resumption of meiosis and cortical granule exocytosis, they did not develop oolemma block to sperm penetration. They could be penetrated by spermatozoa at pronuclear stage and even at the 2-cell stage. This supports the notion that incorporation of sperm plasma membrane into oolemma contributes to the changes in oolemma that block sperm penetration.
Authors:
M Maleszewski; Y Kimura; R Yanagimachi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Molecular reproduction and development     Volume:  44     ISSN:  1040-452X     ISO Abbreviation:  Mol. Reprod. Dev.     Publication Date:  1996 Jun 
Date Detail:
Created Date:  1997-04-23     Completed Date:  1997-04-23     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8903333     Medline TA:  Mol Reprod Dev     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  256-9     Citation Subset:  IM    
Affiliation:
Department of Anatomy and Reproductive Biology, University of Hawaii School of Medicine, Honolulu, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Membrane / physiology*
Female
Humans
Male
Mice
Microinjections
Ovum / physiology*
Sperm-Ovum Interactions / physiology*
Spermatozoa / physiology*
Grant Support
ID/Acronym/Agency:
HD-03402/HD/NICHD NIH HHS

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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