Document Detail


Sperm-damaging effects of electric current: possible role of free radicals.
MedLine Citation:
PMID:  7841663     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The generation of reactive oxygen species (ROS) and sperm damage was evaluated in (a) samples obtained during electroejaculation (EE) of men with spinal cord injury and (b) in electrolyzed HAM's F-10 medium subjected to electric current in vitro. Chemiluminescence data showed a significant increase in ROS in the ejaculates (6 x 10(7) photons/ml) collected immediately after EE and in the electrolyzed medium (3 to 7 x 10(6) photons/ml) when compared to the control (4 to 7 x 10(4) photons/ml). Incubation of normal human sperm with the electrolyzed medium resulted in a significant threefold decrease in percent motility and a twofold decrease in percent viability. Sperm subjected to direct electric stimulation in vitro exhibited a significant twofold decrease in percent motility and percent viability. Superoxide dismutase (SOD) activity decreased significantly in sperm subjected to direct electric current in comparison to the control or the sample incubated with electrolyzed medium. These studies indicate that in vitro and in vivo electrical stimulation generate reactive oxygen species and affect SOD activity, which in part are responsible for decreased sperm motion and viability.
Authors:
M Rajasekaran; W J Hellstrom; R L Sparks; S C Sikka
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Publication Detail:
Type:  Clinical Trial; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Reproductive toxicology (Elmsford, N.Y.)     Volume:  8     ISSN:  0890-6238     ISO Abbreviation:  Reprod. Toxicol.     Publication Date:    1994 Sep-Oct
Date Detail:
Created Date:  1995-03-09     Completed Date:  1995-03-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8803591     Medline TA:  Reprod Toxicol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  427-32     Citation Subset:  IM    
Affiliation:
Department of Urology, Tulane University School of Medicine, New Orleans, LA 70112-2699.
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MeSH Terms
Descriptor/Qualifier:
Analysis of Variance
Chemiluminescent Measurements
Ejaculation
Electric Stimulation / adverse effects*
Free Radicals
Humans
Male
Reactive Oxygen Species*
Sperm Motility / physiology
Spermatozoa / abnormalities*,  enzymology
Spinal Cord Injuries / pathology*
Superoxide Dismutase / metabolism
Chemical
Reg. No./Substance:
0/Free Radicals; 0/Reactive Oxygen Species; EC 1.15.1.1/Superoxide Dismutase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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