Document Detail

Sperm cryopreservation methods.
MedLine Citation:
PMID:  22992915     Owner:  NLM     Status:  In-Data-Review    
There are multiple clinical situations where cryopreservation of sperm is required including sperm banking prior to chemotherapy or radiation treatment for cancer, donor sperm for couples without a male partner, and various etiologies of male factor infertility. Bunge and Sherman presented the first pregnancy resulting from the thawing of cryopreserved sperm in 1953 (Bunge RJ, Nature 172:767-768, 1953). Since that time, cryopreservation techniques have continued to improve. Here, we describe protocols for the cryopreservation and thawing of semen and testicular tissues using a simple liquid nitrogen vapor technique.
Tiffany Justice; Greg Christensen
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  927     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2013  
Date Detail:
Created Date:  2012-09-20     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  209-15     Citation Subset:  IM    
IVF Laboratory, University Women's Healthcare, Louisville, KY, USA.
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