| Sperm cryopreservation methods. | |
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MedLine Citation:
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PMID: 22992915 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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There are multiple clinical situations where cryopreservation of sperm is required including sperm banking prior to chemotherapy or radiation treatment for cancer, donor sperm for couples without a male partner, and various etiologies of male factor infertility. Bunge and Sherman presented the first pregnancy resulting from the thawing of cryopreserved sperm in 1953 (Bunge RJ, Nature 172:767-768, 1953). Since that time, cryopreservation techniques have continued to improve. Here, we describe protocols for the cryopreservation and thawing of semen and testicular tissues using a simple liquid nitrogen vapor technique. |
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Authors:
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Tiffany Justice; Greg Christensen |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 927 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2013 |
Date Detail:
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Created Date: 2012-09-20 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 209-15 Citation Subset: IM |
Affiliation:
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IVF Laboratory, University Women's Healthcare, Louisville, KY, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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