Document Detail


Sperm capacitation in the domestic cat (Felis catus) and leopard cat (Felis bengalensis) as studied with a salt-stored zona pellucida penetration assay.
MedLine Citation:
PMID:  1554505     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The ability of domestic cat or leopard cat spermatozoa to penetrate zonae pellucidae (ZP) of salt-stored, domestic cat oocytes was examined as an assay for sperm capacitation. Ovarian oocytes were recovered after ovariectomy and matured in vitro for 18-36 h. Following removal of cumulus cells, the oocytes were used fresh, or stored (4 degrees C, 0.5-24 weeks) in a HEPES-buffered hypertonic salt solution. Electroejaculated, washed sperm (2-4 x 10(6) sperm/ml) were preincubated for 1.0 h (38 degrees C, 5% CO2 in air) and then co-incubated (2 x 10(5) sperm/ml) with fresh or stored oocytes for 6.0 h. Gametes were incubated in a protein-free, modified Tyrode's solution (TLP-PVA) or in the same medium containing 4.0 mg/ml bovine serum albumin (BSA; TALP-PVA). Treatments were compared for percentage ZP penetration (defined as sperm heads reaching more than halfway through the ZP) as an index of sperm capacitation. In both the domestic cat and leopard cat, there was no difference (P greater than 0.05) in sperm penetration of fresh ZP (domestic cat, 42.5 +/- 5.4%; leopard cat, 38.6 +/- 2.8%) or stored ZP (domestic cat, 32.4 +/- 4.2%; leopard cat, 27.6 +/- 2.3%). Sperm incubated in protein-free medium (TLP-PVA) were less capable (P less than 0.05) of ZP penetration (domestic cat, 14.6 +/- 5.9%; leopard cat, 7.9 +/- 3.0%) than sperm incubated in medium TALP-PVA containing BSA (domestic cat, 60.3 +/- 5.9%; leopard cat, 58.4 +/- 3.0%).(ABSTRACT TRUNCATED AT 250 WORDS)
Authors:
J C Andrews; J G Howard; B D Bavister; D E Wildt
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Molecular reproduction and development     Volume:  31     ISSN:  1040-452X     ISO Abbreviation:  Mol. Reprod. Dev.     Publication Date:  1992 Mar 
Date Detail:
Created Date:  1992-05-04     Completed Date:  1992-05-04     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8903333     Medline TA:  Mol Reprod Dev     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  200-7     Citation Subset:  IM    
Affiliation:
National Zoological Park, Smithsonian Institution, Washington, DC.
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MeSH Terms
Descriptor/Qualifier:
Animals
Carnivora / physiology*
Cats / physiology*
Culture Media, Serum-Free
Female
Isotonic Solutions
Male
Ovum / drug effects,  physiology
Preservation, Biological*
Salts*
Sperm Capacitation / drug effects,  physiology*
Sperm-Ovum Interactions / drug effects,  physiology*
Zona Pellucida / drug effects,  physiology*
Grant Support
ID/Acronym/Agency:
HD23853/HD/NICHD NIH HHS; RR00045/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Culture Media, Serum-Free; 0/Isotonic Solutions; 0/Salts; 0/Tyrode's solution

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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