Document Detail

Spectrum of Bmp5 mutations from germline mutagenesis experiments in mice.
MedLine Citation:
PMID:  9071596     Owner:  NLM     Status:  MEDLINE    
Over 40 years of mutagenesis experiments using the mouse specific-locus test have produced a large number of induced germline mutations at seven loci, among them the short ear locus. We have previously shown that the short ear locus encodes bone morphogenetic protein 5 (BMP5), a member of a large family of secreted signaling molecules that play key roles in axis formation, tissue differentiation, mesenchymalepithelial interactions, and skeletal development. Here we examine 24 chemical- and radiation-induced mutations at the short ear locus. Sequence changes in the Bmp5 open reading frame confirm the importance of cysteine residues in the function of TGF beta superfamily members. The spectrum of N-ethyl-N-nitrosourea-induced mutations also provides new information about the basepair, sequence context, and strand specificity of germline mutations in mammals.
P C Marker; K Seung; A E Bland; L B Russell; D M Kingsley
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Genetics     Volume:  145     ISSN:  0016-6731     ISO Abbreviation:  Genetics     Publication Date:  1997 Feb 
Date Detail:
Created Date:  1997-07-24     Completed Date:  1997-07-24     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0374636     Medline TA:  Genetics     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  435-43     Citation Subset:  IM    
Department of Developmental Biology, Backmen Center B300, Stanford University School of Medicine, Palo Alto, California 94304-5427, USA.
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MeSH Terms
Amino Acid Sequence
Base Sequence
Bone Morphogenetic Proteins / genetics*
DNA, Complementary
Germ-Line Mutation*
Mice, Inbred C3H
Molecular Sequence Data
Polymerase Chain Reaction
Grant Support
1-Y01-ES-50318-00/ES/NIEHS NIH HHS
Reg. No./Substance:
0/Bone Morphogenetic Proteins; 0/DNA, Complementary

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